A THEORY OF OSMOTIC LYSIS OF LIPID VESICLES
A THEORY OF OSMOTIC LYSIS OF LIPID VESICLES
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DOI：
10.1016/s0022-5193(84)80108-3
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发表时间：
1984-01-01
影响因子：
2
通讯作者：
MARKIN, VS
中科院分区：
文献类型：
--
作者：
KOSLOV, MM;MARKIN, VS
当由于渗透压引起的膜膨胀超过其临界值Δ ~ Δ时，囊泡的渗透裂解开始。S，在此膜破裂形成孔。Δ ~的依赖性S上的囊泡半径和各自的渗透压。对于小的（100埃）破裂的囊泡应超过30个大气压，对于大的（10，000埃）囊泡小至10-3 atm。在大（. ltorsim. 1000.膜在合理的时间内破裂时的相对膨胀值仅略微取决于囊泡半径。例如，对于10，000.囊泡占3%。大囊泡的破膜张力约为8达因/厘米。膜张力虽然由于破裂和孔的形成而大大降低，但并不完全消失。它支持引起囊泡（细胞）内容物流出的囊泡内残余压力。同时，发生水通过膜的渗透流入，其导致膜的完全破裂和全部内容物的流出，或其在2、准稳定或脉冲方式中的任一种中的逐渐冲洗。在第一种情况下，孔是稳定开放的，而在第二种情况下，它交替地打开和关闭，每次喷射约5%的内部溶液。分析裂解动力学。最有可能的是脉冲式裂解方案。
Osmotic lysis of vesicles begins when the membrane expansion due to osmotic pressure exceeds its critical value, .DELTA.~S, at which a membrane ruptures to form a pore. The dependence of .DELTA.~S on the vesicle radius and respective osmotic pressures are obtained. The osmotic pressure necessary for small (100 .ANG.) vesicles to rupture should exceed 30 atm, for large (10,000 .ANG.) vesicles it being as small as 10-3 atm. In the case of large (.ltorsim. 1000 .ANG.) vesicles the value of relative expansion of the membrne at which its rupture occurs in a reasonable time only depends slightly on the vesicle radius. For instance, for 10,000 .ANG. vesicles it amounts to 3%. The tension of membrane rupture is about 8 dyn/cm for large vesicles. Membrane tension, although it decreses considerably as a result of rupture and pore formation, does not vanish completely. It supports the residual intravesicular pressure causing the efflux of vesicle (cell) contents. Simultaneously, osmotic influx of water through the membrane occurs that results in either complete rupture of the membrane with the efflux of the whole of the conents, or its gradual washout in either of 2, quasi-steady or pulse-wise regimes. In the first case a pore is steadily open, whereas in the second case it alternately opens and closes, ejecting about 5% of internal solution each time. Lysis kinetics is analyzed. A pulse-wise regime of lysis is most likely.