Differentiation of Potato virus Y strains using improved sets of diagnostic PCR-primers
Differentiation of Potato virus Y strains using improved sets of diagnostic PCR-primers
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DOI：
10.1016/j.jviromet.2006.10.017
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发表时间：
2007-03-01
影响因子：
3.1
通讯作者：
Sztangret-Wisniewska, Joana
中科院分区：
文献类型：
--
作者：
Schubert, Joerg;Fomitcheva, Victoria;Sztangret-Wisniewska, Joana
马铃薯 Y 病毒 (PVY) 是世界范围内最重要的马铃薯病毒之一，有多个毒株群已得到认可。在过去的二十年中，出现了新的 PVY 变体，导致马铃薯块茎出现坏死症状。涉及两组重组菌株：(PVYW)-W-N和PVYNTN，以及NA-PVYNTN。前两种是 PVY-N 株和 O 株之间的重组体，后者是 N 株和未知 PVY 株或其他马铃薯Y病毒株之间的重组体。 PVY 的现有生物学和分子数据表明，PVY 菌株的分类必须进行修订。最近发表的用于基于 RT-PCR 的 PVY 菌株分化的引物存在一些缺陷，因为无法正确识别某些确定的分离株。因此，我们使用最近可用的序列和新生成的 PVY 菌株的完整序列开发了新的引物。这些新开发的引物和程序的可靠性已在近 100 个具有生物学和血清学特征的 PVY 分离株上成功得到证明。 (c) 2006 Elsevier B.V. 保留所有权利。
Potato virus Y (PVY) is one of the most important viruses of potato world-wide, several strain groups are recognized. In the past two decades, novel PVY variants have appeared causing necrotic symptoms on potato tubers. Implicated are two groups of recombinant strains: (PVYW)-W-N and PVYNTN, and NA-PVYNTN. While the first two are recombinants between PVY-N- and O-strains the latter is a recombinant between an N-strain and an unknown PVY strain or other Potyvirus. Available biological and molecular data on PVY suggest that classification of PVY strains has to be revised. Some drawbacks have been found with recently published primers used in RT-PCR based differentiation of PVY strains as some defined isolates could not be identified correctly. Consequently we developed new primers using both recently available sequences and newly generated complete sequences of PVY strains. The reliability of these newly developed primers and procedures was successfully demonstrated on nearly 100 biologically and serologically characterised PVY isolates. (c) 2006 Elsevier B.V. All rights reserved.