SPECIFIC TEMPLATE REQUIREMENTS OF RNA REPLICASES
SPECIFIC TEMPLATE REQUIREMENTS OF RNA REPLICASES
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DOI：
10.1073/pnas.54.2.579
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发表时间：
1965-01-01
影响因子：
11.1
通讯作者：
SPIEGELMAN, S
中科院分区：
文献类型：
--
作者：
HARUNA, I;SPIEGELMAN, S
纯化了在同一宿主中由无关RNA噬菌体诱导的两种RNA复制酶，并检测了它们对不同RNA分子的反应。在最佳离子条件下，两者与异源RNA(包括寄主的核糖体和sRNA)几乎不起作用。这两种复制酶都不能与另一种“S”核糖核酸一起发挥作用。每一种复制酶都识别其起源的核糖核酸基因组，并要求将其作为合成活动的模板。对试图在充满其他核糖核酸分子的细胞环境中引导自己复制的病毒来说，其复制酶的这种有选择性是明显的优势。
Two RNA replicases induced in the same host by unrelated RNA bacteriophages have been purified and their responses to various RNA molecules examined. Under optimal ionic condition1s both are virtually inactive with heterologous RNA, including ribosomal and sRNA of the host. Neither replicase can function with the other''s RNA. Each recognizes the RNA genome of its origin and requires it as a template for synthetic activity. This discriminating selectivity of its replicase is of obvious advantage to a virus attempting to direct its own duplication in a cellular environment replete with other RNA molecules.