Cathepsin expression in oral squamous cell carcinoma: Relationship with clinicopathologic factors
Cathepsin expression in oral squamous cell carcinoma: Relationship with clinicopathologic factors
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DOI：
10.1067/moe.2002.122834
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发表时间：
2002-04-01
影响因子：
--
通讯作者：
Mizuno, A
中科院分区：
文献类型：
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作者：
Kawasaki, G;Kato, Y;Mizuno, A
目标。蛋白水解酶参与了癌细胞的侵袭和转移。在体内，口腔癌细胞容易侵袭骨组织并转移到颌下和颈淋巴结。组织蛋白酶在一些肿瘤组织中的表达可作为预后的指标。本研究的目的是探讨口腔鳞状细胞癌临床病理分级与组织蛋白酶表达的关系，并探讨组织蛋白酶对口腔癌患者预后的影响。用抗组织蛋白酶B、H和L的单抗和抗组织蛋白酶D的多克隆抗体对78例癌组织标本进行免疫组织化学研究。测定血清组织蛋白酶B、H、L和D的活性。对组织蛋白酶的阳性结果进行调查，以确定特定的组织蛋白酶与组织学恶性程度、肿瘤细胞生长、血清组织蛋白酶活性和临床因素之间的任何相关性。组织蛋白B、H、L、D均为阳性。组织蛋白酶B(CB-LI)、组织蛋白酶H(CH-LI)和组织蛋白酶D(CD-LI)的标记指数在癌症组与对照组之间有显著差异(P<0.05)，而组织蛋白酶L(CL-LI)在癌症组与对照组之间差异无统计学意义(P>0.05)。CD-LI与T型(P<；0.05)、CD-LI与增殖细胞核抗原-LI(P<；0.05)密切相关。此外，在TNM分类中，CD-LI和N分类之间存在密切相关(P<；0.05)。病理上，CB-LI和CD-LI与肿瘤的侵袭方式和/或分期密切相关(P<；0.05)。组织蛋白酶D和B的表达与肿瘤的侵袭和进展密切相关。这些蛋白水解酶可能有助于判断口腔癌患者的预后。
Objective. Proteases are involved in the invasion and metastasis of carcinoma cells. In vivo, oral carcinoma cells easily invade the bone tissue and metastasize to the submandibular and neck lymph nodes. Cathepsin expression has been shown in some neoplastic tissues and serves as a prognostic indicator. The purpose of this study was to investigate the relationship between clinicopathohistologic grades and cathepsin expressions in oral squamous cell carcinoma and to investigate which cathepsin provides prognostic information for patients with oral carcinoma.Study design. Immunohistochemical studies were performed on 78 carcinoma samples with monoclonal antibodies against cathepsins B, H, and L, and a polyclonal antibody against cathepsin D. Serial sections were stained by hematoxylin-eosin staining and classified by Anneroth's classification. Cathepsin B, H, L and D activities of blood serum were determined. Positive results indicative of the presence of cathepsin were investigated to determine any correlation between a particular cathepsin and histologic malignancy grades, tumor cell growth, serum cathepsin activities, and clinical factors.Results. Cathepsins B, H, L, and D were positive in every case. Although the labeling indices for cathepsins B (CB-LI), H (CH-LI), and D (CD-LI) for the cancer cases showed significant differences from those of controls, cathepsin L (CL-LI) of cancer cases, showed no difference from that of controls (P < .05). A close correlation was found between CD-LI and T categories of TNM classification (P < .05), and between CD-LI and PCNA-LI (P < .05). Furthermore, a close correlation was found between CD-LI and N categories in TNM classification (P < .05). Pathologically, a close correlation was found between CB-LI or CD-LI and the pattern and/or stage of invasion (P < .05).Conclusion. Cathepsin D and B expression were closely correlated with carcinoma invasion and progression. These proteases may be useful in determining the prognoses of patients with oral carcinoma.