The Dynamic Gene Expression Patterns of Transcription Factors Constituting the Sea Urchin Aboral Ectoderm Gene Regulatory Network
The Dynamic Gene Expression Patterns of Transcription Factors Constituting the Sea Urchin Aboral Ectoderm Gene Regulatory Network
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DOI：
10.1002/dvdy.22514
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发表时间：
2011-01-01
影响因子：
2.5
通讯作者：
Su, Yi-Hsien
中科院分区：
文献类型：
--
作者：
Chen, Jen-Hao;Luo, Yi-Jyun;Su, Yi-Hsien
构建基因调控网络需要了解调控基因的时空表达模式。目前的海胆胚胎外胚层GRN模型包括口（OE）和离口外胚层（AE）的前原肠胚特化功能。与OE不同，OE被分解为几个子域，AE被认为是一个更简单的领域，因为在这个领域缺乏详细的基因表达研究。在这里，我们进行时间和空间的基因表达的八个转录因子基因构成的AE GRN的研究。基于差异基因表达模式，我们得出结论，AE包含至少三个亚结构域在间充质囊胚阶段。我们还对pSmad 1/5/8进行免疫染色以监测BMP信号通路的激活。这些转录因子基因表达模式的动态变化和pSmad 1/5/8的核化可能为解决AE GRN提供了基础。Developmental Dynamics 240：250-260，2011. (C)2010 Wiley-Liss，Inc.
The temporal and spatial expression patterns of regulatory genes are required for building a gene regulatory network (GRN). The current ectoderm GRN model for the sea urchin embryo includes pregastrular specification functions in the oral (OE) and aboral ectoderm (AE). Unlike the OE, which is resolved into several subdomains, the AE is considered a simpler territory due to the lack of detailed gene expression studies in this territory. Here, we perform temporal and spatial gene expression studies on the eight transcription factor genes constituting the AE GRN. Based on the differential gene expression patterns, we conclude that the AE contains at least three subdomains at the mesenchyme blastula stage. We also performed immunostaining for pSmad1/5/8 to monitor the activation of the BMP signaling pathway. The dynamic changes in the expression patterns of these transcription factor genes and the nuclearization of pSmad1/5/8 may provide a foundation for resolving the AE GRN. Developmental Dynamics 240: 250-260, 2011. (C) 2010 Wiley-Liss, Inc.