Protein/DNA interactions in complex DNA topologies: expect the unexpected.
Protein/DNA interactions in complex DNA topologies: expect the unexpected.
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DOI：
10.1007/s12551-016-0208-8
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发表时间：
2016
影响因子：
--
通讯作者：
A Harris, Sarah
中科院分区：
文献类型：
--
作者：
Noy, Agnes;Sutthibutpong, Thana;A Harris, Sarah
DNA 超螺旋会产生紧凑的 DNA 结构，使远端位点变得非常接近。它还会改变 DNA 的局部结构，进而影响药物、其他核酸和蛋白质识别 DNA 的方式。在这里，我们讨论 DNA 超螺旋和复杂 DNA 拓扑的形成如何影响 DNA 识别的热力学。然后，我们使用我们自己的模拟和文献中的例子来推测转录控制和遗传物质的三维组织的影响。我们通过 DNA 超螺旋和拓扑介绍并讨论与分层核结构组织相关的多个长度尺度之间的耦合概念。
DNA supercoiling results in compacted DNA structures that can bring distal sites into close proximity. It also changes the local structure of the DNA, which can in turn influence the way it is recognised by drugs, other nucleic acids and proteins. Here, we discuss how DNA supercoiling and the formation of complex DNA topologies can affect the thermodynamics of DNA recognition. We then speculate on the implications for transcriptional control and the three-dimensional organisation of the genetic material, using examples from our own simulations and from the literature. We introduce and discuss the concept of coupling between the multiple length-scales associated with hierarchical nuclear structural organisation through DNA supercoiling and topology.