CHARACTERIZATION OF THE HUMAN CELL-LINE TE671
CHARACTERIZATION OF THE HUMAN CELL-LINE TE671
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DOI：
10.1093/carcin/10.5.899
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发表时间：
1989-05-01
期刊：
影响因子：
4.7
通讯作者：
COOPER, CS
中科院分区：
文献类型：
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作者：
STRATTON, MR;DARLING, J;COOPER, CS
人髓母细胞瘤细胞系TE671已被广泛用作人髓母细胞瘤的模型。在本研究中，我们已经证明，将该细胞系的DNA导入NIH3T3细胞，发现存在激活的N-ras基因。使用寡核苷酸探针，我们已经证明了N-ras基因是由密码子61的第三个碱基点突变激活的，导致p21ras基因产物中的组氨酸取代谷氨酰胺。我们注意到，这种相对罕见的激活突变也存在于人类横纹肌肉瘤细胞系RD中。基于这一发现，以及观察到TE671的几个表型特征，如肌型烟碱型乙酰胆碱受体和中间丝蛋白desmin的存在，提示肌样起源，我们研究了这两个细胞系的可能相同之处。细胞遗传学分析显示存在TE671和RD共同的标记染色体。使用位点特异性和多位点核心探针的DNA指纹图谱在两个细胞系中显示出难以区分的条带。综上所述，我们的数据表明TE671和RD是同一细胞系的衍生物，我们得出结论，TE671的特性应该归因于横纹肌肉瘤而不是髓母细胞瘤细胞。
The cell line TE671 has been widely used as a model of human medulloblastoma. In the present study we have demonstrated that transfection of DNA from this cell line into NIH 3T3 cells reveals the presence of an activated N-ras gene. Using oligonucleotide probes we have shown that the N-ras gene is activated by a point mutation at the third base of codon 61 resulting in the substitution of histidine for glutamine in the p21 ras gene product. We noted that this relatively uncommon activating mutation is also present in the human rhabdomyosarcoma cell line RD. Based on this finding and on the observation that several of the phenotypic characteristics of TE671, such as the presence of muscle-type nicotinic acetylcholine receptors and the intermediate filament protein desmin, are suggestive of myoid origin we investigated the possible identity of these two cell lines. Cytogenetic analysis revealed the presence of marker chromosomes common to both TE671 and RD. DNA fingerprinting using both locus specific and multilocus core probes showed indistinguishable band patterns in the two cell lines. Taken together our data show that TE671 and RD are derivatives of the same cell line and we conclude that the properties of the TE671 line should be ascribed to rhabdomyosarcoma rather than medulloblastoma cells.