Immunochemical analysis of the vacuolar proton-ATPase B-subunit in the gills of a euryhaline stingray (Dasyatis sabina): effects of salinity and relation to Na(+)/K(+)-ATPase.
Immunochemical analysis of the vacuolar proton-ATPase B-subunit in the gills of a euryhaline stingray (Dasyatis sabina): effects of salinity and relation to Na(+)/K(+)-ATPase.
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广盐黄貂鱼 (Dasyatis sabina) 鳃中液泡质子-ATP 酶 B 亚基的免疫化学分析：盐度的影响以及与 Na( )/K( )-ATP 酶的关系。
DOI：
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发表时间：
2001
影响因子：
2.8
通讯作者：
David H. Evans
中科院分区：
文献类型：
--
作者：
P. Piermarini;David H. Evans
在淡水硬骨鱼的鳃中，液泡质子-ATPase(V-H(+)-ATPase)存在于细胞膜和富含Na(+)/K(+)-ATPase的氯化物细胞上，是摄取和排出H(+)的重要转运体。在棘目鱼类的鳃中，V-H(+)-ATPase还没有得到广泛的研究，也没有检测到它在淡水个体中的表达。本研究的目的是研究环境盐度对大西洋黄貂鱼(Dasyatis Sabina)鳃中V-H(+)-ATPase表达的影响，并确定V-H(+)-ATPase和Na(+)/K(+)-ATPase是否在同一细胞中表达。免疫印迹和免疫组织化学结果表明，淡水黄貂鳃的V-H(+)-ATPase相对丰度最高，富含V-H(+)-ATPase的细胞数量最多。淡水动物海水习服1周后，V-H(+)-ATPase丰度和富含V-H(+)-ATPase的细胞数显著减少。海水环境下的大西洋黄貂鱼V-H(+)-ATPase表达最低，富含V-H(+)-ATPase的细胞数最少。与硬骨鱼不同，淡水黄貂鳃中V-H(+)-ATPase的定位在表层细胞中未发现，而在可能富含线粒体的细胞中出现在基侧膜上。在海水驯化的淡水黄貂鱼和海水黄貂鱼中，V-H(+)-ATPase在细胞质中的定位较强，这可能表明转运蛋白储存在小泡中。应用双重免疫标记技术，我们发现V-H(+)-ATPase和Na(+)/K(+)-ATPase存在于不同的细胞中，这表明在弹性鳃上皮中可能存在两种类型的线粒体丰富的细胞。基于这些发现，我们提出了一个独特的氯化钠和酸碱调节模型，其中富含V-H(+)-ATPase的细胞和富含Na(+)/K(+)-ATPase的细胞分别是Cl(-)摄取/HCO(3)(-)排泄和Na(+)摄取/H(+)排泄的部位。
In the gills of freshwater teleost fishes, vacuolar proton-ATPase (V-H(+)-ATPase) is found on the apical membrane of pavement and chloride (Na(+)/K(+)-ATPase-rich) cells, and is an important transporter for energizing Na(+) uptake and H(+) excretion. In the gills of elasmobranch fishes, the V-H(+)-ATPase has not been extensively studied and its expression in freshwater individuals has not been examined. The goals of this study were to examine the effects of environmental salinity on the expression of V-H(+)-ATPase in the gills of an elasmobranch (the Atlantic stingray, Dasyatis sabina) and determine if V-H(+)-ATPase and Na(+)/K(+)-ATPase are expressed in the same cells. We found that gills from freshwater stingrays had the highest relative abundance of V-H(+)-ATPase and greatest number of V-H(+)-ATPase-rich cells, using immunoblotting and immunohistochemistry, respectively. When freshwater animals were acclimated to sea water for 1 week, V-H(+)-ATPase abundance and the number of V-H(+)-ATPase-rich cells decreased significantly. Atlantic stingrays from seawater environments were characterized by the lowest expression of V-H(+)-ATPase and least number of V-H(+)-ATPase-rich cells. In contrast to teleost fishes, localization of V-H(+)-ATPase in freshwater stingray gills was not found in pavement cells and occurred on the basolateral membrane in cells that are presumably rich in mitochondria. In freshwater stingrays acclimated to sea water and seawater stingrays, V-H(+)-ATPase localization appeared qualitatively to be stronger in the cytoplasm, which may suggest the transporter was stored in vesicles. Using a double-immunolabeling technique, we found that V-H(+)-ATPase and Na(+)/K(+)-ATPase occurred in distinct cells, which suggests there may be two types of mitochondrion-rich cells in the elasmobranch gill epithelium. Based on these findings, we propose a unique model of NaCl and acid-base regulation where the V-H(+)-ATPase-rich cells and Na(+)/K(+)-ATPase-rich cells are the sites of Cl(-) uptake/HCO(3)(-) excretion and Na(+) uptake/H(+) excretion, respectively.
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DOI：
10.1152/ajprenal.1985.249.4.f553
发表时间：
1985
期刊：
The American journal of physiology
影响因子：
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作者：
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1992-04-15
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1999-02
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发表时间：
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期刊：
The Journal of experimental biology
影响因子：
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