ESTABLISHMENT OF A CLONED LINE OF LEWIS LUNG-CARCINOMA CELLS ADAPTED TO CELL-CULTURE
ESTABLISHMENT OF A CLONED LINE OF LEWIS LUNG-CARCINOMA CELLS ADAPTED TO CELL-CULTURE
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DOI：
10.1016/0304-3835(80)90130-5
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发表时间：
1980-01-01
期刊：
影响因子：
9.7
通讯作者：
JANIK, P
中科院分区：
文献类型：
--
作者：
BERTRAM, JS;JANIK, P
从刘易斯肺癌中分离出一个适合培养的克隆细胞系，命名为刘易斯肺癌细胞系1（LLC 1）。它在补充有2%胎牛血清的RPMI 1640培养基中作为单层培养物生长，平板接种效率为apxx。94%，倍增时间为21 h。LLC 1细胞在C57 B1小鼠中保持高度致瘤性，并产生原发性肿瘤和肺转移，在组织学上与原始肿瘤系无法区分。对于肿瘤质量为apx的来源于LLC 1细胞的皮下肿瘤的倍增时间为23小时。0.1 g和40小时，肿瘤质量为aptx。1克的该细胞系在塑料基质上形成离散集落，并可用于病灶测定以确定药物诱导的细胞毒性。报告了一些化疗药物的结果;一般来说，体外测量的敏感性与已发表的刘易斯肺癌体内敏感性报告不一致。
A cloned line of cells adapted to culture was isolated from the Lewis lung carcinoma and was designated the Lewis lung carcinoma line 1 (LLC1). It grows as a monolayer culture in RPMI 1640 medium supplemented with 2% fetal calf serum with a plating efficiency of .apprx. 94% and a doubling time of 21 h. LLC1 cells remain highly tumorigenic in C57B1 mice and produce primary tumors and lung metastases histologically indistinguishable from the original tumor line. The doubling time for a subcutaneous tumor derived from LLC1 cells was 23 h for a tumor mass of .apprx. 0.1 g and 40 h for a tumor mass of .apprx. 1 g. The cell line forms discrete colonies on a plastic substrate and can be used in a focus assay to determine drug induced cytotoxicity. Results with a number of chemotherapeutic agents are reported; in general, sensitivity measured in vitro does not correspond with published reports of sensitivity of the Lewis lung carcinoma in vivo.