Molecular cloning and sequencing of the cDNA encoding the bat CD4
Molecular cloning and sequencing of the cDNA encoding the bat CD4
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DOI：
10.1016/j.vetimm.2005.12.002
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发表时间：
2006-06-15
影响因子：
1.8
通讯作者：
Yoshikawa, Yasuhiro
中科院分区：
文献类型：
--
作者：
Omatsu, Tsutomu;Nishimura, Yorihiro;Yoshikawa, Yasuhiro
翼手目被认为是各种病原微生物的载体或天然储存库。然而，对翼手目免疫系统的基础研究很少。这是首次报道蝙蝠CD4基因的cDNA序列测定。与其他动物的CD4比较和系统发育分析表明，蝙蝠CD4与猫和狗的同源性高于与人和小鼠的同源性。此外，从CD4 Ig样C型I区的结构分析，在蝙蝠CD4中有18个额外的氨基酸插入。此外，蝙蝠CD4缺乏半胱氨酸，这表明不能形成二硫键。人、猴和小鼠的CD4含有半胱氨酸和二硫键，但猪、猫、鲸和狗的CD4和蝙蝠一样，缺少半胱氨酸。我们进行了本研究，以帮助阐明传染病来自蝙蝠以及蝙蝠免疫系统。(c)2006 Elsevier B.V.保留所有权利。
Chiroptera is thought to be a vector or a natural reservoir of various pathogenic microbes. However, there are few basic studies on the subject of chiroptera immune systems. This is the first report to determine the sequence of bat CD4 cDNA. Comparison with other animals' CD4 and phylogenetic analysis have shown that bat CD4 had a higher homology to cat and dog CD4 than to human and mouse CD4. Moreover, from the analysis of the structure of the CD4 Ig-like C-type I region, in bat CD4 there was an insertion of 18 extra amino acids. In addition, bat CD4 lacked cystein, which suggested that the disulfide bond could not be formed. Human, monkey and mouse CD4 have the cystein and the disulfide bond, but pig, cat, whale and dog CD4, like that of the bat, lacked the cystein. We conducted the present study in order to help elucidate the infectious diseases derived from the bat as well as bat immune systems. (c) 2006 Elsevier B.V. All rights reserved.