Incomplete transformation of rat cells by a deletion mutant of adenovirus type 5
Incomplete transformation of rat cells by a deletion mutant of adenovirus type 5
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5型腺病毒缺失突变体对大鼠细胞的不完全转化
DOI：
10.1128/jvi.38.3.1048-1054.1981
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发表时间：
1981
影响因子：
5.4
通讯作者：
H. Shimojo
中科院分区：
文献类型：
--
作者：
K. Shiroki;K. Maruyama;I. Saito;Y. Fukui;H. Shimojo
大鼠3Y1细胞用5型腺病毒（Ad5）野生型、dl312（删除1.5-4.5个图谱单位之间的902个碱基对）和dl313（删除3.5-10.5个图谱单位之间的2,350个碱基对）感染。培养4周后，野生型和dl313感染的细胞中出现转化灶。在 dl312 和模拟感染的细胞中没有观察到焦点。 dl313 诱导的病灶密度低于野生型诱导的病灶密度。从 dl313 诱导灶建立的细胞系（313Y 细胞）包含 dl313 基因组的 E1 基因（仅 E1a）。从 Ad5 野生型诱导的灶建立的细胞系（5WY 细胞）含有野生型的 E1 基因（E1a 和 b）。 313Y细胞和5WY细胞中E1基因转录模式的差异与dl313和野生型感染细胞中的差异相同。接种5WY细胞的软琼脂培养物中形成集落，但接种313Y细胞后未形成集落。与5WY细胞相比，313Y细胞的转化表型不完整。在非生长的 3Y1 细胞中，dl313 和 Ad5 野生型诱导细胞 DNA 合成，但 dl312 则不然。上述结果表明，E1a基因在dl313感染的细胞中起作用，但在dl312感染的细胞中不起作用，并且诱导细胞DNA合成和细胞转化等功能依赖于Ad5 E1a基因的表达。
Rat 3Y1 cells were infected with adenovirus type 5 (Ad5) wild type, dl312 (deletion of 902 base pairs between 1.5-4.5 map units), and dl313 (deletion of 2,350 base pairs between 3.5-10.5 map units). After cultivation for 4 weeks, transformed foci appeared in wild type- and dl313-infected cells. No focus was observed in dl312- and mock-infected cells. Foci induced by dl313 were less dense than those induced by wild type. Cell lines (313Y cells) established from dl313-induced foci contained the E1 gene of the dl313 genome (E1a only). Cell lines (5WY cells) established from Ad5 wild type-induced foci contained the E1 gene of wild type (E1a and b). The difference between the transcriptional patterns of the E1 gene in 313Y cells and that in 5WY cells was the same as the difference in dl313- and wild type-infected cells. Colonies were formed in soft agar culture inoculated with 5WY cells, but no colony was formed after inoculation of 313Y cells. The transformed phenotype of 313Y cells was incomplete compared with that in 5WY cells. In nongrowing 3Y1 cells, dl313 and Ad5 wild type induced cellular DNA synthesis but dl312 did not. The above results suggest that the E1a gene is functioning in dl313-infected but not in dl312-infected cells and that such functions as induction of cellular DNA synthesis and transformation of cells are dependent on expression of the Ad5 E1a gene.