Improvements in ethanol production from xylose by mating recombinant xylose-fermenting Saccharomyces cerevisiae strains
Improvements in ethanol production from xylose by mating recombinant xylose-fermenting Saccharomyces cerevisiae strains
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DOI：
10.1007/s00253-012-3914-6
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发表时间：
2012-06-01
影响因子：
5
通讯作者：
Kondo, Akihiko
中科院分区：
文献类型：
--
作者：
Kato, Hiroko;Suyama, Hiroaki;Kondo, Akihiko
为了提高重组酿酒酵母菌株利用木质纤维素原料的半纤维素组分的能力，需要提高木糖转化为乙醇的效率。在本研究中，木糖发酵，单倍体，酵母细胞的相反的交配型杂交产生一个二倍体菌株窝藏两套木糖同化基因编码木糖还原酶，木糖醇脱氢酶，木酮糖激酶。与其亲本菌株MT 8 - 1X 405和NBRC 1440 X相比，杂交菌株MN 8140 XX的乙醇产量分别提高了1.3倍和1.9倍。木糖消耗率和乙醇生产率也通过杂交得到提高。这项研究表明，发酵能力的改善是由于染色体加倍以及木糖同化基因拷贝数的增加。此外，与亲本菌株相比，MN 8140 XX菌株在升高的温度（38 A ° C）和酸性条件（pH 3.8）下表现出更高的乙醇产量。因此，简单的杂交技术促进了木糖发酵活性的增加。
To improve the ability of recombinant Saccharomyces cerevisiae strains to utilize the hemicellulose components of lignocellulosic feedstocks, the efficiency of xylose conversion to ethanol needs to be increased. In the present study, xylose-fermenting, haploid, yeast cells of the opposite mating type were hybridized to produce a diploid strain harboring two sets of xylose-assimilating genes encoding xylose reductase, xylitol dehydrogenase, and xylulokinase. The hybrid strain MN8140XX showed a 1.3- and 1.9-fold improvement in ethanol production compared to its parent strains MT8-1X405 and NBRC1440X, respectively. The rate of xylose consumption and ethanol production was also improved by the hybridization. This study revealed that the resulting improvements in fermentation ability arose due to chromosome doubling as well as the increase in the copy number of xylose assimilation genes. Moreover, compared to the parent strain, the MN8140XX strain exhibited higher ethanol production under elevated temperatures (38 A degrees C) and acidic conditions (pH 3.8). Thus, the simple hybridization technique facilitated an increase in the xylose fermentation activity.