Spectral Responses in Zebrafish Horizontal Cells Include a Tetraphasic Response and a Novel UV-Dominated Triphasic Response
Spectral Responses in Zebrafish Horizontal Cells Include a Tetraphasic Response and a Novel UV-Dominated Triphasic Response
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DOI：
10.1152/jn.00644.2009
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发表时间：
2010-11-01
影响因子：
2.5
通讯作者：
Nelson, Ralph
中科院分区：
文献类型：
--
作者：
Connaughton, Victoria P.;Nelson, Ralph
康诺顿副总裁，纳尔逊R。斑马鱼水平细胞的光谱响应包括一个四相响应和一个新的UV主导的三相响应。神经生理学杂志104：2407-2422，2010。首次发表于2010年7月7日; doi：10.1152/jn.00644.2009。斑马鱼是具有红色（R，570 nm）、绿色（G，480 nm）、蓝色（B，415 nm）和UV（U，362 nm）视锥细胞的四色体。虽然其他鲤科动物视网膜中的神经元具有丰富的颜色处理神经回路，但作为脊椎动物色觉的遗传模型，斑马鱼视网膜中单个神经元的光谱响应还有待研究。使用染料填充的尖锐微电极，水平细胞电压响应于不同波长和辐照度的光刺激记录在灌流的眼杯。光谱特性进行了定性和定量评估。水平细胞的六个光谱类别被区分。两种单相反应类型（L1和L2）在所有波长下均超极化。L1灵敏度在493 nm处达到峰值，接近G锥吸光度最大值。模拟光谱表明，同样加权的输入从R和G锥，此外，一个“隐藏的双折射”从蓝色锥。这些被分类为R-/G-/（B+）。L2灵敏度在R视锥吸收峰附近的563 nm处最大;建模光谱以R视锥为主，G视锥贡献较小。B和UV锥信号很小或不存在。这些是R-/g-。四色（C型）水平细胞的去极化（+）或超极化（-）取决于刺激波长。这些类型是双相（R+/G-/B-），在467 nm处具有峰值激发，在G和B视锥吸收峰之间，UV三相（r-/G+/U-），在362 nm处具有峰值激发，类似于UV视锥，以及蓝色三相（r-/G +/B-/u-）和蓝色四相（r-/G+/B-/u+），分别在409和411 nm处具有峰值激发，类似于B视锥。紫外线三相和蓝色四相水平细胞光谱响应是独特的，并没有预期在以前的模型中的远端颜色电路在鲤。
Connaughton VP, Nelson R. Spectral responses in zebrafish horizontal cells include a tetraphasic response and a novel UV-dominated triphasic response. J Neurophysiol 104: 2407-2422, 2010. First published July 7, 2010; doi:10.1152/jn.00644.2009. Zebrafish are tetra-chromats with red (R, 570 nm), green (G, 480 nm), blue (B, 415 nm), and UV (U, 362 nm) cones. Although neurons in other cyprinid retinas are rich in color processing neural circuitry, spectral responses of individual neurons in zebrafish retina, a genetic model for vertebrate color vision, are yet to be studied. Using dye-filled sharp microelectrodes, horizontal cell voltage responses to light stimuli of different wavelengths and irradiances were recorded in a superfused eyecup. Spectral properties were assessed both qualitatively and quantitatively. Six spectral classes of horizontal cell were distinguished. Two monophasic response types (L1 and L2) hyperpolarized at all wavelengths. L1 sensitivities peaked at 493 nm, near the G cone absorbance maximum. Modeled spectra suggest equally weighted inputs from both R and G cones and, in addition, a "hidden opponency" from blue cones. These were classified as R-/G-/(b+). L2 sensitivities were maximal at 563 nm near the R cone absorbance peak; modeled spectra were dominated by R cones, with lesser G cone contributions. B and UV cone signals were small or absent. These are R-/g-. Four chromatic (C-type) horizontal cells were either depolarized (+) or hyperpolarized (-) depending on stimulus wavelength. These types are biphasic (R+/G-/B-) with peak excitation at 467 nm, between G and B cone absorbance peaks, UV triphasic (r-/G+/U-) with peak excitation at 362 nm similar to UV cones, and blue triphasic (r-/G+/B-/u-) and blue tetraphasic (r-/G+/B-/u+), with peak excitation at 409 and 411 nm, respectively, similar to B cones. UV triphasic and blue tetraphasic horizontal cell spectral responses are unique and were not anticipated in previous models of distal color circuitry in cyprinids.