LIPID-METABOLISM IN CHLAMYDIA TRACHOMATIS-INFECTED CELLS - DIRECTED TRAFFICKING OF GOLGI-DERIVED SPHINGOLIPIDS TO THE CHLAMYDIAL INCLUSION
LIPID-METABOLISM IN CHLAMYDIA TRACHOMATIS-INFECTED CELLS - DIRECTED TRAFFICKING OF GOLGI-DERIVED SPHINGOLIPIDS TO THE CHLAMYDIAL INCLUSION
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DOI：
10.1073/pnas.92.11.4877
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发表时间：
1995-05-23
影响因子：
11.1
通讯作者：
ROCKEY, DD
中科院分区：
文献类型：
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作者：
HACKSTADT, T;SCIDMORE, MA;ROCKEY, DD
沙眼衣原体在不与溶酶体融合的未表征的胞内囊泡内经历其整个生命周期。（4-硝基苯并-2-氧杂-1，3-二唑）]}氨基己酰基鞘氨醇（C-6-NBD-Cer），结合常规荧光或共聚焦显微镜以鉴定高尔基体和衣原体包涵体之间的相互作用，我们不仅观察到高尔基体和衣原体包涵体之间的密切物理联系，而且还观察到与衣原体本身相关的这种荧光探针的代谢物的最终存在，从C-6-NBD-Cer内源性合成的鞘磷脂被特异性运输到包涵体并掺入细胞内衣原体的细胞壁中，布雷菲德菌素A可抑制衣原体对荧光鞘脂的掺入。因此，衣原体占据一个囊泡远端的高尔基体，接受顺行囊泡交通从高尔基体通常绑定的质膜，总的来说，数据表明，衣原体夹杂物可能代表一个独特的隔室内的trans-Golgi网络。
Chlamydia trachomatis undergoes its entire life cycle within an uncharacterized intracellular vesicle that does not fuse with lysosomes, We used a fluorescent Golgi-specific probe, {N-[7-(4-nitrobenzo-2-oxa-1,3-diazole)]}aminocaproylsphingosine (C-6-NBD-Cer), in conjunction with conventional fluorescence or confocal microscopy to identify interactions between the Golgi apparatus and the chlamydial inclusion, We observed not only a close physical association between the Golgi apparatus and the chlamydial inclusion but the eventual presence of a metabolite of this fluorescent probe associated with the chlamydiae themselves, Sphingomyelin, endogenously synthesized from C-6-NBD-Cer, was specifically transported to the inclusion and incorporated into the cell wall of the intracellular chlamydiae, Incorporation of the fluorescent sphingolipid by chlamydiae was inhibited by brefeldin A. Chlamydiae therefore occupy a vesicle distal to the Golgi apparatus that receives anterograde vesicular traffic from the Golgi normally bound for the plasma membrane, Collectively, the data suggest that the chlamydial inclusion may represent a unique compartment within the trans-Golgi network.