The cacao procyanidin extract-caused anti-hyperglycemic effect was changed by the administration timings
The cacao procyanidin extract-caused anti-hyperglycemic effect was changed by the administration timings
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DOI：
10.3164/jcbn.20-45
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发表时间：
2020-07-01
影响因子：
2.4
通讯作者：
Yamashita, Yoko
中科院分区：
文献类型：
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作者：
Hironao, Ken-yu;Ashida, Hitoshi;Yamashita, Yoko
哺乳动物的生物钟大约有24小时的节律。包括葡萄糖代谢在内的能量代谢受生物钟的调节。葡萄糖代谢不仅受进食量及其能量的影响，而且还受进餐时间的影响。我们已报道可可液中富含原花青素的提取物(CLPR)通过AMP激活的蛋白激酶途径改善餐后高血糖。然而，CLPR给药时机对餐后高血糖的影响及其信号转导途径尚不清楚。在本研究中，我们比较了静止期(光期)和活动期(暗期)给予CLPR对糖代谢的影响。ICR小鼠在静止期(而不是活动期)单独口服CLPR，可促进AMP激活的蛋白激酶及其上游肝激酶B1的磷酸化，并促进葡萄糖转运蛋白4到骨骼肌质膜的转位，从而降低餐后高血糖。这些结果表明，在静息期摄入CLPR更有效地抑制了餐后高血糖。
Mammals have the biological clocks with approximately 24 h rhythm. Energy metabolism including glucose metabolism is regulated by the biological clocks. Glucose metabolism is affected by not only meal volume and its energy but also meal timing. We have reported that cacao liquor procyanidin-rich extract (CLPr) ameliorated the postprandial hyperglycemia through AMP-activated protein kinase pathway. However, the effect of administration timing of CLPr on the postprandial hyperglycemia and its signaling pathway are still unclear. In the present study, we compared the effect of CLPr-administration at the rest-phase (light-period) and active-phase (dark-period) on glucose metabolism. Single oral administration of CLPr to ICR mice at the rest-phase, but not at the active-phase, promoted phosphorylation of AMP-activated protein kinase and its upstream liver kinase B1 and translocation of glucose transporter 4 to the plasma membrane in the skeletal muscle, resulting in reduced postprandial hyperglycemia. These results indicated that the intake of CLPr at the rest-phase more effectively suppressed postprandial hyperglycemia.