The effect of orphanin FQ on the expression of NMDAR1 after cerebral ischemic injury in rats
The effect of orphanin FQ on the expression of NMDAR1 after cerebral ischemic injury in rats
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发表时间：
2010
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通讯作者：
Wang Zheng-bi
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作者：
Wang Zheng-bi
目的研究孤儿素FQ（OFQ）对大鼠脑缺血损伤后n -甲基- d -天冬氨酸受体1（NMDAR1）表达的影响。方法40只健康成年雄性Sprague-Dawley大鼠，采用膜内螺纹法建立大脑中动脉闭塞模型，并干扰脑室内注射OFQ。TUNEL法检测神经元凋亡，免疫组化法检测NMDAR1的表达。结果脑缺血损伤后神经元凋亡细胞数量增加，NMDAR1表达增加（P0.05）。OFQ治疗后，神经元凋亡细胞数及NMDAR1表达均较缺血组显著增加（P0.05），且与OFQ剂量有关。结论OFQ可通过上调NMDAR1的表达增强脑缺血氧化损伤。
Objective To study the effect of orphanin FQ(OFQ)on the expression of N-methyl-D-asparate receptor 1(NMDAR1)after cerebral ischemic injury in rats. Methods Forty adult healthy male Sprague-Dawley rats were established into middle cerebral artery occlusion model by intralumial thread method,and interfered with intraventricular injection of OFQ. The neuronal apoptosis was counted by TUNEL assay and the expression of NMDAR1 was determined by immunohistochemical assay. Results The number of neuronal apoptotic cells and the expression of NMDAR1 increased following cerebral ischemic injury(P0.05). After treatment with OFQ,the number of neuronal apoptotic cells and the expression of NMDAR1 increased significantly than those in the ischemic group(P0.05),and were releated with the OFQ dosage. Conclusion OFQ could enhance the oxide injury induced by cerebral ischemia through upregulating the expression of NMDAR1.