Perturbation of spermine synthase Gene Expression and Transcript Profiling Provide New Insights on the Role of the Tetraamine Spermine in Arabidopsis Defense against Pseudomonas viridiflava
Perturbation of spermine synthase Gene Expression and Transcript Profiling Provide New Insights on the Role of the Tetraamine Spermine in Arabidopsis Defense against Pseudomonas viridiflava
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DOI：
10.1104/pp.110.171413
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发表时间：
2011-08-01
期刊：
影响因子：
7.4
通讯作者：
Luis Pieckenstain, Fernando
中科院分区：
文献类型：
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作者：
Elisa Gonzalez, Maria;Marco, Francisco;Luis Pieckenstain, Fernando
利用拟南芥（Arabidopsis thaliana）-绿黄假单胞菌（Pseudomonasviridiflava）致病系统研究了四胺精胺在植物防御病原菌中的作用。在转基因植物（35 S：：精胺合酶[SPMS]），过表达的SPMS基因和积累精胺，以及在spms突变体与低精胺水平的干扰植物精胺水平对细菌感染的易感性的影响进行了评估。前者表现出比野生型植株更高的抗性，而后者则更感病。外源精胺的野生型植物的供应也增加了抗病性。多胺氧化酶抑制剂SL-11061部分抵消了精胺提供的抗性增加，表明精胺的保护作用部分取决于其氧化。此外，通过转录谱分析35 S：：SPMS-9和spms-2植物，分析了精胺水平扰动引起的基因表达的总体变化。与野生型相比，在35 S：：SPMS-9植物中检测到602个基因的过表达，而312个基因被下调。在spms-2系中，分别有211个和158个基因表达上调和下调。基因本体术语富集分析表明，许多基因仅在35 S：：SPMS-9中过表达，参与病原体感知和防御反应。值得注意的是，几个家族的抗病基因，转录因子，激酶，核苷酸和DNA/RNA结合蛋白在这个线过表达。因此，许多精胺响应基因可能参与抗P. viridiflava的鉴定。这些结果支持了精胺参与植物对绿黄单胞菌抗性的观点。
The role of the tetraamine spermine in plant defense against pathogens was investigated by using the Arabidopsis (Arabidopsis thaliana)-Pseudomonas viridiflava pathosystem. The effects of perturbations of plant spermine levels on susceptibility to bacterial infection were evaluated in transgenic plants (35S:: spermine synthase [SPMS]) that overexpressed the SPMS gene and accumulated spermine, as well as in spms mutants with low spermine levels. The former exhibited higher resistance to P. viridiflava than wild-type plants, while the latter were more susceptible. Exogenous supply of spermine to wild-type plants also increased disease resistance. Increased resistance provided by spermine was partly counteracted by the polyamine oxidase inhibitor SL-11061, demonstrating that the protective effect of spermine partly depends on its oxidation. In addition, global changes in gene expression resulting from perturbations of spermine levels were analyzed by transcript profiling 35S:: SPMS-9 and spms-2 plants. Overexpression of 602 genes was detected in 35S:: SPMS-9 plants, while 312 genes were down-regulated, as compared to the wild type. In the spms-2 line, 211 and 158 genes were up-and down-regulated, respectively. Analysis of gene ontology term enrichment demonstrated that many genes overexpressed only in 35S:: SPMS-9 participate in pathogen perception and defense responses. Notably, several families of disease resistance genes, transcription factors, kinases, and nucleotide-and DNA/ RNA-binding proteins were overexpressed in this line. Thus, a number of spermine-responsive genes potentially involved in resistance to P. viridiflava were identified. The obtained results support the idea that spermine contributes to plant resistance to P. viridiflava.