Early photon tomography allows fluorescence detection of lung carcinomas and disease progression in mice in vivo
Early photon tomography allows fluorescence detection of lung carcinomas and disease progression in mice in vivo
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DOI：
10.1073/pnas.0804798105
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发表时间：
2008-12-09
影响因子：
11.1
通讯作者：
Ntziachristos, Vasilis
中科院分区：
文献类型：
--
作者：
Niedre, Mark J.;de Kleine, Ruben H.;Ntziachristos, Vasilis
靶向荧光探针的成像为研究动物模型体内的疾病和组织功能提供了显著的优势。相反，宏观层析成像是具有挑战性的，因为光在生物组织中的高散射和重建数学的病态性质。在这项工作中，我们使用最早传输的光子通过Lewis肺癌小鼠，从而大大减少了组织散射的影响。通过使用对半胱氨酸蛋白酶敏感的荧光探针，与传统方法相比，该方法获得了出色的成像性能。不仅在原发肿瘤中，而且在器官水平上与癌症进展和炎症反应相关的周围组织中，实现了生化异常的准确可视化。这些发现经组织学和离体荧光显微镜证实。成像保真度证明了一种方法，可以使用广泛的荧光探针来准确地可视化整个动物体内细胞和分子水平的事件。
Imaging of targeted fluorescent probes offers significant advantages for investigating disease and tissue function in animal models in vivo. Conversely, macroscopic tomographic imaging is challenging because of the high scatter of light in biological tissue and the ill-posed nature of the reconstruction mathematics. In this work, we use the earliest-transmitted photons through Lewis Lung Carcinoma bearing mice, thereby dramatically reducing the effect of tissue scattering. By using a fluorescent probe sensitive to cysteine proteases, the method yielded outstanding imaging performance compared with conventional approaches. Accurate visualization of biochemical abnormalities was achieved, not only in the primary tumor, but also in the surrounding tissue related to cancer progression and inflammatory response at the organ level. These findings were confirmed histologically and with ex vivo fluorescence microscopy. The imaging fidelity demonstrated underscores a method that can use a wide range of fluorescent probes to accurately visualize cellular- and molecular-level events in whole animals in vivo.