Effects of herbivory on internal ethylene production and sex expression in Cucurbita texana
Effects of herbivory on internal ethylene production and sex expression in Cucurbita texana
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DOI：
10.1046/j.1365-2435.2000.00413.x
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发表时间：
2000-04-01
影响因子：
5.2
通讯作者：
Stephenson, AG
中科院分区：
文献类型：
--
作者：
Krupnick, GA;Avila, G;Stephenson, AG
1. 在一项研究中使用了田间种植的 Cucurbita texana，以确定受损叶子产生的应激乙烯是否会促进性别表达的变化。2。通过将充满乙烯的注射器插入距树枝生长尖端七个节点的节间的中空室中，通过实验提高内部乙烯浓度。乙烯增强的枝条比对照枝条产生明显更多的雌花蕾，并且在花发育后期经历了更多的芽败育。3．使用纸打孔器（从所有叶子中去除 15%）模拟叶甲甲虫叶子损害的时间和程度。在整个生长季节每3天对新生长物应用模拟食草。4．在实验的第二周和第三周期间新生长受损后1天以及在第四周和第五周期间受损后2天，受损植物中与生长尖端相邻的节间恢复的内源乙烯浓度显着高于未受损植物。到第六周，没有明显的差异。5．受损植物比未受损植物产生更少的雌花芽，表明来自模拟食草动物的应激乙烯不会诱导雌性。在受损过程中可能会产生抑制雌花芽产生的第二信使。
1. Field-grown Cucurbita texana was used in a study to determine if stress ethylene from damaged leaves promotes changes in sex expression.2. Internal ethylene concentrations were experimentally enhanced by inserting an ethylene-filled syringe into the hollow chamber of an internode seven nodes from the growing tip of a branch. Branches enhanced with ethylene produced significantly more pistillate buds than control branches and experienced greater bud abortion later in floral development.3. The timing and magnitude of Diabrotica beetle leaf damage was simulated using a paper-hole puncher (15% removed from all leaves). Simulated herbivory was applied to new growth every 3 days throughout the growing season.4. Endogenous ethylene concentrations recovered from the internode adjacent to the growing tip were significantly greater in damaged plants than undamaged plants 1 day after damage to new growth during the second and third week of the experiment, and 2 days after damage during the fourth and fifth week. By the sixth week, no significant differences were evident.5. Damaged plants produced fewer pistillate buds than undamaged plants, indicating that stress ethylene from simulated herbivory does not induce femaleness. A second messenger that suppresses pistillate bud production may be produced during damage.