Cloning and expression of hyperglycemic (CHH) and molt-inhibiting (MIH) hormones mRNAs from the eyestalk of shrimps of Litopenaeus vannamei grown in different temperature and salinity conditions
Cloning and expression of hyperglycemic (CHH) and molt-inhibiting (MIH) hormones mRNAs from the eyestalk of shrimps of Litopenaeus vannamei grown in different temperature and salinity conditions
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DOI：
10.1016/j.aquaculture.2007.04.014
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发表时间：
2007-09-28
期刊：
影响因子：
4.5
通讯作者：
Munoz, Ma. Enriqueta
中科院分区：
文献类型：
--
作者：
Lago-Leston, Asuncion;Ponce, Elizabeth;Munoz, Ma. Enriqueta
本研究描述了温度和盐度对从凡纳滨对虾幼鱼获得的 chh 和 mih 基因表达的影响。结果表明，温度对基因表达的影响比盐度更大。从暴露于 20 摄氏度的虾的眼柄中获得了两种不同的 mih 转录本。直接序列证据表明，这两种亚型来自以选择性剪接方式转录的 mih 基因。基因组-cDNA 比对表明，mih-1 包含 4 个外显子，产生 73 个氨基酸的成熟 MIH 样肽 (Liv-MIH-1)，而 mih-2 包含外显子 1、11 和 TV，产生74 个残基的成熟 MIH 样肽 (Liv-MIH-2)。 chh 转录物被认为由 3 个外显子组成，并产生 74 个氨基酸的成熟肽 (Liv-CHH)。 mih 和 chh 基因的外显子-内含子边界遵循剪接供体和受体位点的 Chambon 规则 (GT-AG)。基因结构和系统发育分析表明，这三种肽与 CHH 组的相关性比与 MIH/GIH 组的相关性更高。正如在其他 CHH 家族成员中观察到的那样，Liv-MIH 和 Liv-MIH-2 可能具有多功能作用。 (c) 2007 Elsevier B.V. 保留所有权利。
This study describes the effect of temperature and salinity on the expression of chh and mih genes obtained from Litopenaeus vannamei juveniles. Results suggest that temperature had a higher effect on gene expression than salinity. Two different mih transcripts were obtained from the eyestalks of shrimps exposed to 20 degrees C. Direct sequence evidence suggests that these two isoforms come from a mih gene transcribed in an alternative splicing manner.The genome-cDNA alignment suggests that the mih-1 contains 4 exons and gives rise to a mature MIH-like peptide of 73 amino acids (Liv-MIH-1), while mih-2 contains exons 1, 11 and TV and gives rise to a mature MIH-like peptide of 74 residues (Liv-MIH-2). The chh transcript is suggested to consist of 3 exons and gives rise to a mature peptide of 74 amino acids (Liv-CHH). The exon-intron boundary of the mih and chh genes follows Chambon's rule (GT-AG) for the splice donor and acceptor sites. Gene structure and phylogenetic analysis imply that these three peptides are more related to the CHH group than to the MIH/GIH group. Liv-MIH and Liv-MIH-2 may have a multi-functional role as observed in other CHH family members. (c) 2007 Elsevier B.V. All rights reserved.