DIRECT IN-VIVO EVIDENCE THAT D2 DOPAMINE-RECEPTORS CAN MODULATE DOPAMINE UPTAKE
DIRECT IN-VIVO EVIDENCE THAT D2 DOPAMINE-RECEPTORS CAN MODULATE DOPAMINE UPTAKE
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DOI：
10.1016/0304-3940(94)90096-5
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发表时间：
1994-08-01
影响因子：
2.5
通讯作者：
GERHARDT, GA
中科院分区：
文献类型：
--
作者：
CASS, WA;GERHARDT, GA
采用体内电化学方法确定局部应用雷氯必利（一种 D2 受体拮抗剂）和 SCH-23390（一种 D1 受体拮抗剂）对大鼠纹状体、伏核和内侧前额皮质局部应用多巴胺清除的影响。使用 Nafion 涂层的单碳纤维电极以 5 Hz 连续进行计时电流记录。当校准量的多巴胺以 5 分钟的间隔从邻近电极（280-310μm）的微量移液器压力喷射时，在所有三个区域中检测到瞬态和可再现的多巴胺信号。在压力喷射多巴胺之前，从第二个微量移液管局部应用雷氯必利，增加了多巴胺信号的幅度和时程，表明多巴胺转运蛋白受到显着抑制。相反，局部应用SCH-23390或盐水对多巴胺信号没有影响。这些数据表明，D2（而非 D1）多巴胺受体可以调节多巴胺转运蛋白的活性。
In vivo electrochemistry was used to determine the effects of locally applied raclopride (a D2 receptor antagonist) and SCH-23390 (a D1 receptor antagonist) on the clearance of locally applied dopamine in the striatum, nucleus accumbens, and medial prefrontal cortex of rats. Chronoamperometric recordings were continuously made at 5 Hz using Nafion-coated, single carbon fiber electrodes. When a calibrated amount of dopamine was pressure ejected at 5-min intervals from a micropipette adjacent (280-310 mu m) to the electrode, transient and reproducible dopamine signals were detected in all three regions. Local application of raclopride from a second micropipette, prior to pressure ejection of dopamine, increased the amplitude and time course of the dopamine signals, indicating significant inhibition of the dopamine transporter. In contrast, local application of SCH-23390 or saline had no effect on the dopamine signals. These data indicate that D2, but not D1, dopamine receptors can modulate the activity of the dopamine transporter.