Cloning, characterization, and production of a novel lysozyme by different expression hosts.
Cloning, characterization, and production of a novel lysozyme by different expression hosts.
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DOI：
10.4014/jmb.1404.04039
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发表时间：
2014-07
影响因子：
2.8
通讯作者：
Hai-feng Zhang;Gang Fu;Dawei Zhang
中科院分区：
文献类型：
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作者：
Hai-feng Zhang;Gang Fu;Dawei Zhang
溶菌酶是一种存在于鸡蛋白色、眼泪、唾液和其他分泌物中的蛋白质。作为防腐剂的天然替代品，溶菌酶可以作为天然抗生素。本研究从土壤中分离到一株地衣芽孢杆菌TIB 320，它含有一个具有多种功能的溶菌酶基因。我们克隆并在大肠杆菌中表达了溶菌酶。杆菌抑菌试验表明，该溶菌酶对几种标准菌株具有较广的抑菌谱。溶菌酶在pH 3 ~ 9和20 ~ 60 ℃范围内均能保持较高的活性。溶菌酶在40 ℃时对胃蛋白酶和胰蛋白酶有一定的抗性。通过使用多种表达策略在B中优化了溶菌酶的生产。枯草杆菌WB 800。来自B的溶菌酶。地衣TIB 320作为食品或饲料添加剂将是有前途的。
Lysozyme is a protein found in egg white, tears, saliva, and other secretions. As a marketable natural alternative to preservatives, lysozyme can act as a natural antibiotic. In this study, we have isolated Bacillus licheniformis TIB320 from soil, which contains a lysozyme gene with various features. We have cloned and expressed the lysozyme in E. coli. The antimicrobial activity of the lysozyme showed that it had a broad antimicrobial spectrum against several standard strains. The lysozyme could maintain efficient activities in a pH range between 3 and 9 and from 20°C to 60°C, respectively. The lysozyme was resistant to pepsin and trypsin to some extent at 40°C. Production of the lysozyme was optimized by using various expression strategies in B. subtilis WB800. The lysozyme from B. licheniformis TIB320 will be promising as a food or feed additive.