Detection of N6-methyladenosine in SARS-CoV-2 RNA by methylated RNA immunoprecipitation sequencing.
Detection of N6-methyladenosine in SARS-CoV-2 RNA by methylated RNA immunoprecipitation sequencing.
复制标题
甲基化RNA免疫沉淀测序检测SARS-CoV-2 RNA中n6 -甲基腺苷。
DOI：
10.1016/j.xpro.2021.101067
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发表时间：
2022-03-18
期刊：
影响因子：
--
通讯作者：
Rana TM
中科院分区：
文献类型：
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作者：
Li N;Rana TM
腺苷n6甲基化（m6A）是最丰富的内部mRNA修饰，是基因表达的重要转录后调控因子。在这里，我们描述了一种甲基化RNA免疫沉淀测序（MeRIP-Seq）方案，以检测和量化严重急性呼吸综合征冠状病毒2 (SARS-CoV-2) RNA中的m6A修饰。该协议针对低病毒RNA水平进行了优化，并易于适用于其他应用。有关使用和执行本协议的完整详情，请参阅。用MeRIP-Seq分析SARS-CoV-2病毒RNA中m6A修饰的方法MeRIP-RT-qPCR生物信息学分析和验证方法n6 -甲基化腺苷（m6A）是最丰富的内部mRNA修饰，是基因表达的重要转录后调节因子。在这里，我们描述了一种甲基化RNA免疫沉淀测序（MeRIP-Seq）方案，以检测和量化严重急性呼吸综合征冠状病毒2 (SARS-CoV-2) RNA中的m6A修饰。该协议针对低病毒RNA水平进行了优化，并易于适用于其他应用。
N6-methylation of adenosine (m6A) is the most abundant internal mRNA modification and is an important post-transcriptional regulator of gene expression. Here, we describe a protocol for methylated RNA immunoprecipitation sequencing (MeRIP-Seq) to detect and quantify m6A modifications in severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA. The protocol is optimized for low viral RNA levels and is readily adaptable for other applications. For complete details on the use and execution of this protocol, please refer to. Procedure to generate stable cell lines for SARS-CoV-2 viral infection Protocol for profiling m6A modifications in SARS-CoV-2 viral RNA by MeRIP-Seq Procedures for bioinformatics analysis and validation by MeRIP-RT-qPCR N6-methylation of adenosine (m6A) is the most abundant internal mRNA modification and is an important post-transcriptional regulator of gene expression. Here, we describe a protocol for methylated RNA immunoprecipitation sequencing (MeRIP-Seq) to detect and quantify m6A modifications in severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA. The protocol is optimized for low viral RNA levels and is readily adaptable for other applications.