In early OA, thinning of the subchondral plate is directly related to cartilage damage: results from a canine ACLT-meniscectomy model
In early OA, thinning of the subchondral plate is directly related to cartilage damage: results from a canine ACLT-meniscectomy model
复制标题
DOI：
10.1016/j.joca.2010.01.004
复制
发表时间：
2010-05-01
影响因子：
7
通讯作者：
Mastbergen, S. C.
中科院分区：
文献类型：
--
作者：
Intema, F.;Hazewinkel, H. A. W.;Mastbergen, S. C.
目的：骨关节炎（OA）的发病机制包括软骨退变、滑膜炎症和骨质改变。慢慢地，这些特征的顺序和相互关系变得越来越清晰。OA的早期模型提示除了骨小梁变化之外，软骨下板变薄。在本研究中，在犬前交叉韧带横断（ACLT）-骨切除模型中研究了软骨下骨的变化。该模型的特点是关节内的变异性相对于软骨损伤（主要是内侧）和加载（外侧卸载由于轴移位）。方法：在13只拉布拉多犬，OA引起的前交叉韧带和切除内侧半月板的横断。12周后，使用改良的Mankin评分（0-11）对软骨完整性进行组织学评价，并通过Alcian Blue测定法测定蛋白多糖含量。结果：胫骨内侧间室损伤严重（Mankin评分+3.5，糖胺聚糖（GAG）含量-28%），外侧间室损伤轻微（Mankin评分+1.6，GAG含量-15%）。软骨下板变薄和多孔仅出现在内侧（分别为-21%，+87%）。有趣的是，骨小梁结构的变化几乎没有发生在内侧室（体积分数-7%），但在外侧室（-20%.结论：变薄的软骨下板是一个局部现象有关的软骨退变，而骨小梁的变化与机械（卸载）负荷。在设计和解释OA治疗中干扰骨转换的研究时，应考虑导致OA骨变化的不同机制。(C)2010年国际骨关节炎研究学会。由爱思唯尔有限公司出版。保留所有权利。
Objective: The pathogenesis of osteoarthritis (OA) includes cartilage degeneration, synovial inflammation, and bone changes. Slowly, the sequence and inter-relationship of these features is becoming clearer. Early models of OA suggest thinning of the subchondral plate in addition to trabecular bone changes. In the present study subchondral bone changes were studied in the canine anterior cruciate ligament transection (ACLT)-meniscectomy model. This model is characterized by intra-joint variability with respect to cartilage damage (predominantly medial) and loading (lateral unloading due to a shifted axis).Methods: In 13 Labrador dogs, OA was induced by transection of the anterior cruciate ligament and removal of the medial meniscus. Twelve weeks later, cartilage integrity was evaluated histologically using the modified Mankin score (0-11), and proteoglycan content was determined by Alcian Blue assay. Bone architecture of the tibia was quantified by micro-CT.Results: Cartilage damage was severe in the medial compartment (Mankin score +3.5, glycosaminoglycan (GAG) content -28%) and mild in the lateral compartment (Mankin score +1.6, GAG content -15%). Thinning and porosity of the subchondral plate were only present on the medial side (-21%, +87%, respectively). Interestingly, changes in trabecular bone structure did almost not occur in the medial compartment (volume fraction -7%) but were clear in the lateral compartment (-20%).Conclusion: Thinning of the subchondral plate is a localized phenomenon related to cartilage degeneration while trabecular bone changes are related to mechanical (un)loading. The different mechanisms responsible for bone changes in OA should be taken in account when designing and interpreting studies interfering with bone turnover in the treatment of OA. (C) 2010 Osteoarthritis Research Society International. Published by Elsevier Ltd. All rights reserved.