Differentiation of monocytes into dendritic cells in a model of transendothelial trafficking
Differentiation of monocytes into dendritic cells in a model of transendothelial trafficking
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DOI：
10.1126/science.282.5388.480
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发表时间：
1998-10-16
期刊：
影响因子：
56.9
通讯作者：
Muller, WA
中科院分区：
文献类型：
--
作者：
Randolph, GJ;Beaulieu, S;Muller, WA
对于抗原呈递细胞的树突状细胞系统来说，必不可少的是隐蔽的树突状细胞，它们穿过传入淋巴进入淋巴结，在那里它们发起免疫反应。20年前发现的遮盖细胞的起源尚不清楚。含内皮细胞培养的单核细胞在2天内分化为树突状细胞，尤其是吞噬内皮下胶原颗粒后。这些新生的树突状细胞在内皮上以腔-腔方向迁移，就像进入淋巴管时所发生的那样。留在内皮下基质中的单核细胞成为巨噬细胞。因此，单核细胞有两种与不同的迁移模式相关的潜在命运。
Essential to the dendritic cell system of antigen-presenting cells are the veiled dendritic cells that traverse afferent Lymph to enter lymph nodes, where they initiate immune responses. The origin of veiled cells, which were discovered 20 years ago, is unclear. Monocytes cultured with endothelium differentiated into dendritic cells within 2 days, particularly after phagocytosing particles in subendothelial collagen. These nascent dendritic cells migrated across the endothelium in the ablumenal-to-lumenal direction, as would occur during entry into Lymphatics. Monocytes that: remained in the subendothelial matrix became macrophages. Therefore, monocytes have two potential fates associated with distinct patterns of migration.