Lipid monolayers: why use half a membrane to characterize protein-membrane interactions?
Lipid monolayers: why use half a membrane to characterize protein-membrane interactions?
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DOI：
10.1016/s0959-440x(99)80061-x
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发表时间：
1999-08-01
影响因子：
6.8
通讯作者：
Brockman, H
中科院分区：
文献类型：
--
作者：
Brockman, H
膜活性蛋白和肽的变体越来越多地通过合成和分子工程获得。当确定这些蛋白质与脂质膜的相互作用后的结构变化的影响时，在空气-水界面处的脂质的单分子膜比双层和其他脂质分散体具有显著的优势。在过去的一年中，各种蛋白质-脂质相互作用的特点已成功地使用相对简单的表面测量。
Variants of membrane-active proteins and peptides are increasingly available through synthesis and molecular engineering. When determining the effects of structural changes upon the interaction of these proteins with lipid membranes, monomolecular films of lipids at the air-water interface have significant advantages over bilayers and other lipid dispersions. In the past year, a variety of protein-lipid interactions has been characterized successfully using relatively simple surface measurements.