THE USE OF ANTIAVIDIN ANTIBODY AND AVIDIN - BIOTIN - PEROXIDASE COMPLEX IN IMMUNOPEROXIDASE TECHNIQUES
THE USE OF ANTIAVIDIN ANTIBODY AND AVIDIN - BIOTIN - PEROXIDASE COMPLEX IN IMMUNOPEROXIDASE TECHNIQUES
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DOI：
10.1093/ajcp/75.6.816
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发表时间：
1981-01-01
影响因子：
3.5
通讯作者：
FANGER, H
中科院分区：
文献类型：
--
作者：
HSU, SM;RAINE, L;FANGER, H
亲和素对小分子维生素生物素具有非凡的亲和力。将生物素或亲和素共价偶联到过氧化物酶分子上，不会干扰它们的正常生化功能。亲和素或生物素分子，无论是过氧化物酶偶联的还是非偶联的，可以通过抗亲和素抗体被带到抗原位置。介绍了几种基于这一原理的免疫组织化学染色技术。利用亲和素-生物素-过氧化物酶复合体的方法比未标记抗体(PAP)法更灵敏。该方法包括4个步骤：一抗(羊抗人抗原)、二抗(兔抗羊免疫球蛋白)、羊抗生物素抗体、抗生物素-过氧化物酶复合体。文中还讨论了该技术在慢性甲状腺炎患者血清免疫球蛋白检测中的应用。
Avidin has an extraordinary affinity for the small molecule vitamin biotin. Covalently coupling biotin or avidin to peroxidase molecules does not interfere with their normal biochemical functions. The avidin or biotin molecules, either peroxidase conjugated or unconjugated, can be brought to the antigen sites by means of an antiavidin antibody. Several immunohistochemical staining techniques based on this principal were described. The method utilizing an avidin-biotin-peroxidase complex was more sensitive than the unlabeled antibody (PAP) method. This method involved 4 sequential staining procedures: primary antibody (goat anti-human antigen); secondary antibody (rabbit antigoat IgG) added in relative excess; goat antiavidin antibody; avidin-biotin-peroxidase complex. The applications of this technique [as for IgG plasma cell detection in human chronic thyroiditis] are discussed.