Immunomodulation by fish-oil containing lipid emulsions in murine acute respiratory distress syndrome.
Immunomodulation by fish-oil containing lipid emulsions in murine acute respiratory distress syndrome.
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DOI：
10.1186/cc13850
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发表时间：
2014-04-29
期刊：
影响因子：
--
通讯作者：
Mayer K
中科院分区：
文献类型：
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作者：
Hecker M;Ott J;Sondermann C;Schaefer M;Obert M;Hecker A;Morty RE;Vadasz I;Herold S;Rosengarten B;Witzenrath M;Seeger W;Mayer K
急性呼吸窘迫综合征（ARDS）是重症监护病房死亡的主要原因。 ARDS 患者通常需要以脂肪乳剂为主要成分的肠外营养。除了作为能量供应外，这些脂肪乳剂还可能对肺部完整性和炎症表现出不同的调节作用。在先发制人的策略中，我们研究了三种不同的静脉注射脂肪乳剂对 ARDS 小鼠模型中肺形态、白细胞侵袭、蛋白质渗漏和细胞因子的影响。在脂多糖（LPS）攻击之前，小鼠接受生理盐水溶液、纯长链甘油三酯（LCT）乳剂、含有中链甘油三酯（MCT）的混合乳剂（LCT/MCT）或含有鱼油（FO）的混合乳剂（LCT/MCT/FO）的输注。与接受 LCT 或 LCT/MCT 的小鼠相比，预先注射含鱼油脂肪乳剂的小鼠在 LPS 攻击后显示出白细胞侵袭、蛋白质渗漏、髓过氧化物酶活性和肺泡腔细胞因子产生减少。与这些发现一致，在 LCT/MCT/FO 组中，LPS 诱导的肺损伤后通过组织学染色评估的肺形态改善得更快。关于上述参数，在输注LCT或LCT与MCT组合的小鼠之间没有观察到显着差异。含鱼油的脂肪乳剂可能在急性肺损伤的小鼠模型中发挥抗炎和促消退作用。因此，用 n-3 脂肪酸部分替代 n-6 脂肪酸可能对需要肠外营养的有 ARDS 风险的危重患者有益。
Acute respiratory distress syndrome (ARDS) is a major cause of mortality in intensive care units. Patients with ARDS often require parenteral nutrition with lipid emulsions as essential components. Besides being an energy supply, these lipid emulsions might display differential modulatory effects on lung integrity and inflammation. In a pre-emptive strategy, we investigated the impact of three different intravenously infused lipid emulsions on lung morphology, leukocyte invasion, protein leakage and cytokines in a murine model of ARDS. Mice received an infusion of normal saline solution, a pure long-chain triglycerides (LCT) emulsion, a medium-chain triglycerides (MCT) containing mixed emulsion (LCT/MCT), or a fish oil (FO) containing mixed emulsion (LCT/MCT/FO) before lipopolysaccharide (LPS) challenge. Mice pre-infused with fish oil-containing lipid emulsion showed decreased leukocyte invasion, protein leakage, myeloperoxidase activity, and cytokine production in their alveolar space after LPS challenge compared to mice receiving LCT or LCT/MCT. In line with these findings, lung morphology assessed by histological staining after LPS-induced lung injury improved faster in the LCT/MCT/FO group. Concerning the above mentioned parameters, no significant difference was observed between mice infused with LCT or the combination of LCT and MCT. Fish oil-containing lipid emulsions might exert anti-inflammatory and pro-resolving effects in the murine model of acute lung injury. Partial replacement of n-6 fatty acids with n-3 fatty acids may thus be of benefit for critically ill patients at risk for ARDS which require parenteral nutrition.