The effect of ethylene glycol methacrylate phosphate in PEG hydrogels on mineralization and viability of encapsulated hMSCs
The effect of ethylene glycol methacrylate phosphate in PEG hydrogels on mineralization and viability of encapsulated hMSCs
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DOI：
10.1016/j.biomaterials.2005.08.014
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发表时间：
2006-03-01
期刊：
影响因子：
14
通讯作者：
Anseth, KS
中科院分区：
文献类型：
--
作者：
Nuttelman, CR;Benoit, DSW;Anseth, KS
由于其对蛋白质吸附的抗性，聚（乙二醇）（PEG）水凝胶不能支持人间充质干细胞（hMSCs）的粘附。人骨髓间充质干细胞通过细胞粘附蛋白粘附于各种基质，骨桥蛋白是骨髓间充质干细胞和成骨细胞在骨中发现的一种重要的细胞粘附蛋白。因此，我们假设细胞粘附到PEG水凝胶可以通过积极促进整个水凝胶中矿物相的形成而显著改善。这是使用光反应性的含磷酸盐分子乙二醇甲基丙烯酸酯磷酸盐（EGMP）完成的。当EGMP被纳入到PEG水凝胶，它导致骨样矿物相的形成，使用组成分析和X-射线衍射验证。此外，在EGMP存在下，凝胶包封的hMSC的细胞活力从EGMP不存在下的15%增加到50 mm EGMP存在下的97%。细胞活力的这种改善被认为是由于矿化水凝胶隔离细胞分泌的骨桥蛋白的能力。结果发现，含有EGMP的PEGDA水凝胶能够螯合重要的细胞粘附蛋白骨桥蛋白，并且这些水凝胶促进hMSC粘附和铺展。(c)2005爱思唯尔有限公司保留所有权利。
Owing to their resistance to protein adsorption, poly(ethylene glycol) (PEG) hydrogels are unable to support the adhesion of human mesenchymal stem cells (hMSCs). Human MSCs attach to various substrates through cell adhesion proteins, and osteopontin is an important cell adhesion protein for both MSCs and osteoblasts found in bone. As such, we hypothesized that cell adhesion to a PEG hydrogel could be dramatically improved by actively promoting the formation of a mineral phase throughout the hydrogel. This was accomplished using the photoreactive, phosphate-containing molecule ethylene glycol methacrylate phosphate (EGMP). When EGMP was incorporated into the PEG hydrogel, it resulted in the formation of a bone-like mineral phase, as verified using compositional analysis and X-ray diffraction. Furthermore, cell viability of gel-encapsulated hMSCs was increased in the presence of EGMP from 15% in the absence of EGMP to 97% in the presence of 50 mm EGMP. This improvement in cell viability was thought to be due to the ability of mineralized hydrogels to sequester cell-secreted osteopontin. It was found that EGMP-containing PEGDA hydrogels are able to sequester the important cell adhesion protein osteopontin, and these hydrogels promoted hMSC adhesion and spreading. (c) 2005 Elsevier Ltd. All rights reserved.