TREP, a novel protein necessary for gliding motility of the malaria sporozoite
TREP, a novel protein necessary for gliding motility of the malaria sporozoite
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DOI：
10.1016/j.ijpara.2008.10.004
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发表时间：
2009-03-01
影响因子：
4
通讯作者：
Menard, Robert
中科院分区：
文献类型：
--
作者：
Combe, Audrey;Moreira, Cristina;Menard, Robert
原生动物顶复合体门寄生虫的入侵阶段具有穿越宿主组织和侵入宿主细胞的能力，使用一种称为滑翔运动的独特运动类型。滑翔运动是由亚膜肌动蛋白-肌球蛋白马达驱动的，马达产生的力通过顶端复合体特异性血栓响应蛋白相关匿名蛋白（TRAP）家族的跨膜蛋白传导到寄生虫表面。这些蛋白具有短的细胞质尾部，通过糖酵解酶醛缩酶与肌动蛋白-肌球蛋白马达相互作用。疟原虫是由蚊子传播给哺乳动物宿主的疟原虫的一个阶段，其孢子虫的滑行运动取决于TRAP蛋白。我们描述了第二种蛋白质，在这里被称为TREP，它也在疟原虫孢子的滑动运动中起作用。TREP是一种跨膜蛋白，具有TRAP蛋白家族成员典型的短细胞质尾部，以及包含单个血栓反应蛋白I型重复结构域的大细胞外区域。TREP转录本主要在卵囊期孢子体中表达。携带TREP基因被破坏的伯氏疟原虫孢子体入侵蚊子唾液腺的能力大大降低，并且在滑翔运动方面表现出严重缺陷。我们得出结论，蚊子体内疟原虫孢子体的滑行运动至少取决于两种蛋白质：TRAP和TREP。(C) 2008澳大利亚寄生虫学学会Elsevier Ltd.出版。版权所有。
The invasive stages of parasites of the protozoan phylum Apicomplexa have the capacity to traverse host tissues and invade host cells using a unique type of locomotion called gliding motility. Gliding motility is powered by a sub-membranous actin-myosin motor, and the force generated by the motor is transduced to the parasite surface by transmembrane proteins of the apicomplexan-specific thrombospondin-related anonymous protein (TRAP) family. These proteins possess short cytoplasmic tails that interact with the actin-myosin motor via the glycolytic enzyme aldolase. Gliding motility of the Plasmodium sporozoite, the stage of the malaria parasite that is transmitted by the mosquito to the mammalian host, depends on the TRAP protein. We describe a second protein, herein termed TREP, which also plays a role in the gliding motility of the Plasmodium sporozoite. TREP is a transmembrane protein that possesses a short cytoplasmic tail typical of members of the TRAP family of proteins, as well as a large extracellular region that contains a single thrombospondin type I repeat domain. TREP transcripts are expressed predominantly in oocyst stage sporozoites. Plasmodium berghei sporozoites harbouring a disrupted TREP gene have a highly diminished capacity to invade mosquito salivary glands and display a severe defect in gliding motility. We conclude that the gliding motility of the Plasmodium sporozoite in the mosquito depends on at least two proteins, TRAP and TREP. (C) 2008 Australian Society for Parasitology Inc. Published by Elsevier Ltd. All rights reserved.