Circulating plasma cells detected by flow cytometry as a predictor of survival in 302 patients with newly diagnosed multiple myeloma
Circulating plasma cells detected by flow cytometry as a predictor of survival in 302 patients with newly diagnosed multiple myeloma
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DOI：
10.1182/blood-2005-05-1858
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发表时间：
2005-10-01
期刊：
影响因子：
20.3
通讯作者：
Rajkumar, SV
中科院分区：
文献类型：
--
作者：
Nowakowski, GS;Witzig, TE;Rajkumar, SV
应用流式细胞术检测302例初诊多发性骨髓瘤（MM）患者外周血中的浆细胞（PC），以CD 38（+）CD 45（-）细胞为门控。报告了每50 000个单核细胞中循环PC的数量。在80例（27%）患者中，未观察到循环PC; 106例（35%）患者有1 - 10例，115例（38%）患者有10例以上循环PC。302例患者的中位总生存期为47个月。循环PC少于10个的患者中位生存期为58.7个月，而循环PC多于10个的患者中位生存期为37.3个月（P = 0.001）。在多变量分析中，循环PC的预后价值与β 2-微球蛋白、白蛋白和C反应蛋白无关。肿瘤质量和循环PC之间只有微弱的相关性，表明循环PC的出现可能是肿瘤生物学的反映。我们的结论是，在新诊断的MM患者中通过流式细胞术测量的循环PC的数量是生存的独立预测因子。
We detected circulating plasma cells (PCs) by flow cytometry in 302 patients with newly diagnosed multiple myeloma (MM) by gating on CD38(+)CD45(-) cells. The number of circulating PCs per 50 000 mononuclear cells was reported. In 80 (27%) patients, no circulating PC were seen; 106 (35%) patients had 1 to 10 and 115 (38%) patients had more than 10 circulating PCs. Median overall survival for the 302 patients was 47 months. Patients with 10 or fewer circulating PCs had a median survival of 58.7 months, whereas patients with more than 10 circulating PCs had a median survival of 37.3 months (P =.001). On Multivariate analysis, the prognostic value of circulating PCs was independent of beta(2)-microglobulin, albumin, and C-reactive protein. There was only a weak correlation between tumor mass and circulating PCs, suggesting that the appearance of circulating PCs may be a reflection of tumor biology. We conclude that the number of circulating PCs measured by flow cytometry in patients with newly diagnosed MM is an independent predictor of survival.