Reproductive Cost Associated With Juvenile Hormone in Bt-Resistant Strains of Helicoverpa armigera (Lepidoptera: Noctuidae)
Reproductive Cost Associated With Juvenile Hormone in Bt-Resistant Strains of Helicoverpa armigera (Lepidoptera: Noctuidae)
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DOI：
10.1093/jee/tow233
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发表时间：
2016-12
影响因子：
2.2
通讯作者：
W. N. Zhang;L. Ma;B. J. Wang;L. Chen;M. Khaing;Y. Lu;G. Liang;Y. Guo
中科院分区：
文献类型：
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作者：
W. N. Zhang;L. Ma;B. J. Wang;L. Chen;M. Khaing;Y. Lu;G. Liang;Y. Guo
摘要转Bt基因作物在害虫防治中的作用日益显著，但目标害虫的抗性发展是Bt作物可持续利用的主要问题。在采取避难所策略时，目标害虫的抗性适应度成本被认为是延缓抗性的主要因素之一。对棉铃虫Helicoverpaarmigera（Hübner，1809）3个独立种群的生活史特征进行了比较，结果表明，抗性个体的后代数量少，繁殖力低，卵孵化率低，成虫寿命长。抗性品系繁殖力差与成熟卵泡数量、卵巢重量比和最长卵巢小管长度下降有关。有趣的是，保幼激素（JH）水平出现较高的抗性品系相对于敏感品系。体内应用甲氧普烯（JH类似物）可有效降低受精率和孵化率，并可上调检测到的JH效价。这些结果表明，对Bt毒素的抗性降低了H. JH水平在棉铃虫生殖能力与Bt抗性之间的权衡中起重要作用。
Abstract Transgenic Bacillus thuringiensis (Bt) crops are increasingly significant in pest control, but resistance development of target pests is a major issue in the sustainable deployment of Bt crops. The fitness cost of resistance in target pests is regarded as one of the main factors delaying resistance when adopting the refuge strategy. In this study, we compared the life-history traits of three independent sets of Helicoverpa armigera (Hübner, 1809) adults, of each there were a susceptible population and a Cry1Ac-resistant population derived by selection from it. Confirming to the previous studies, resistant individuals exhibited fewer progeny, less fecundity, lower egg hatching rate, and longer adult longevity. And poor fecundity in resistant strains was associated with the decline of the mature follicular amount, the ovarian weight ratio, and the length of the longest ovarian tubule. Interestingly, the juvenile hormone (JH) level appeared higher in resistant strains relative to susceptible strains. Application of methoprene (JH analogue) in vivo was effective in reducing fecundity and hatchability with the up-regulation of detected JH titer. These results suggested that resistance against Bt toxin reduced the reproductive capacity of H. armigera, and JH level is affected in the tradeoff between reproductive capacity and Bt resistance.