THE DROSOPHILA 74EF EARLY PUFF CONTAINS E74, A COMPLEX ECDYSONE-INDUCIBLE GENE THAT ENCODES 2 ETS-RELATED PROTEINS
THE DROSOPHILA 74EF EARLY PUFF CONTAINS E74, A COMPLEX ECDYSONE-INDUCIBLE GENE THAT ENCODES 2 ETS-RELATED PROTEINS
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DOI：
10.1016/0092-8674(90)90217-3
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发表时间：
1990-04-06
期刊：
影响因子：
64.5
通讯作者：
HOGNESS, DS
中科院分区：
文献类型：
--
作者：
BURTIS, KC;THUMMEL, CS;HOGNESS, DS
我们已经从果蝇多线染色体的74 EF位置分离出蜕皮激素诱导基因E74。我们发现，E74由三个嵌套的转录单位，来自独特的启动子，但共享一个单一的多聚腺苷酸化位点。60 kb的E74 A单位被蜕皮激素直接诱导，并导致6.0 kb的mRNA的合成，该mRNA包含一个异常长的5“前导序列（1891个核苷酸），具有17个短的ORF。在七个E74 A内含子的第五个内是两个E74 B启动子，其指导4.8和5.1 kb mRNA的合成。这些转录单位的嵌套排列导致两个E74蛋白的形成，每个蛋白具有连接到共同C-末端结构域的独特N-末端结构域。独特的N-末端结构域含有富含酸性氨基酸的区域，而C-末端结构域富含碱性氨基酸，并且与ets原癌基因超家族编码的蛋白质非常相似。
We have isolated an ecdysone-inducible gene, E74, from the early puff at position 74EF in the Drosophila polytene chromosomes. We show that E74 consists of three nested transcription units that derive from unique promoters but share a single polyadenylation site. The 60 kb E74A unit is directly induced by ecdysone and leads to the synthesis of a 6.0 kb mRNA that contains an unusually long 5'' leader (1891 nucleotides) with 17 short ORFs. Within the fifth of the seven E74A introns are two E74B promoters that direct the synthesis of 4.8 and 5.1 kb mRNAs. The nested arrangement of these transcription units leads to the formation of two E74 proteins, each with a unique N-terminal domain joined to a common C-terminal domains. The unique N-terminal domains contain regions rich in acidic amino acids while the C-terminal domain is rich in basic amino acids and is very similar to proteins encoded by the ets proto-oncogene superfamily.