Distances of tyrosine residues from a spin-label hapten in the combining site of a specific monoclonal antibody.
Distances of tyrosine residues from a spin-label hapten in the combining site of a specific monoclonal antibody.
复制标题
特定单克隆抗体结合位点中酪氨酸残基与自旋标记半抗原的距离。
DOI：
10.1021/bi00317a041
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发表时间：
1984
期刊：
影响因子：
2.9
通讯作者：
McConnell,HM
中科院分区：
文献类型：
--
作者：
Anglister,J;Frey,T;McConnell,HM
材料和方法已经描述了自旋标记半抗原的合成（Balakrishnan等人，1982). AN 02细胞系的起源、维持和标记已经在先前描述（Anglister等人，1984））。通过标准程序制备Fab片段。在室温下，在PBS中，自旋标记半抗原结合的平衡稳定常数为4 × 106 mol/L。1在相同条件下，DNP-Gly的稳定常数为2 × 106 mol/L。
Materials and MethodsThe synthesis of the spin-label hapten has been described (Balakrishnan et al., 1982).The origin, maintenance, and labeling of the AN02 cell line have been described previously (Anglister et al., 1984)). Fab fragments were prepared by standard procedures. The equilibrium stability constant for the binding of the spin-label hapten is 4 X 106 mol/L at room temperature in PBS. 1 The stability constant of DNP-Gly is 2 X 106 mol/L under the same conditions.