Hyper response to ovarian stimulation affects the follicular fluid metabolomic profile of women undergoing IVF similarly to polycystic ovary syndrome
Hyper response to ovarian stimulation affects the follicular fluid metabolomic profile of women undergoing IVF similarly to polycystic ovary syndrome
复制标题
DOI：
10.1007/s11306-018-1350-z
复制
发表时间：
2018-04-01
期刊：
影响因子：
3.6
通讯作者：
Lo Turco, Edson Guimaraes
中科院分区：
文献类型：
--
作者：
Cordeiro, Fernanda Bertuccez;Cataldi, Thais Regiani;Lo Turco, Edson Guimaraes
在体外受精（IVF）过程中，对控制性卵巢刺激（COS）的高反应是诊断为多囊卵巢综合征（PCOS）的患者的共同特征，尽管未诊断的患者也可能表现出这种反应。本研究分析了接受IVF的患者的卵泡液（FF）样本。方法从PCOS和IVF期间的高反应患者获得FF样本PCOS组（N = 15）、无PCOS但IVF期间有高反应的患者（HR组，N = 44）和接受IVF的正常反应患者（对照组，N = 22）。FF样品进行Bligh和Dyer提取，然后通过超高效液相色谱质谱法进行代谢组学分析，考虑两次技术重复。临床资料采用方差分析和卡方检验进行分析。代谢组学数据采用多元统计分析，生物标志物的显著性采用方差分析。结果临床数据显示卵泡产量、卵母细胞和胚胎质量存在差异。在15种拟定的生物标志物中，14种在对照组中丰度增加，并归因于脂肪酸、甘油二酯、甘油三酯、神经酰胺、磷酸神经酰胺、磷脂酰胆碱和鞘磷脂。PCOS患者表现出增加丰度的代谢产物的m/z 144.0023，这是不归因于一个class.Conclusion的临床和代谢的相似性，观察到的FF的高反应者与PCOS诊断和不表明共同的生物标志物，可以帮助辅助工具的发展，用于评估IVF参数。
Introduction During in vitro fertilization (IVF), the hyper response to controlled ovarian stimulation (COS) is a common characteristic among patients diagnosed with polycystic ovary syndrome (PCOS), although non-diagnosed patients may also demonstrate this response.Objectives In an effort to investigate follicular metabolic characteristics associated with hyper response to COS, the present study analyzed follicular fluid (FF) samples from patients undergoing IVF.Methods FF samples were obtained from patients with PCOS and hyper response during IVF (PCOS group, N = 15), patients without PCOS but with hyper response during IVF (HR group, N = 44), and normo-responder patients receiving IVF (control group, N = 22). FF samples underwent Bligh and Dyer extraction, followed by metabolomic analysis by ultra-performance liquid chromatography mass spectrometry, considering two technical replicates. Clinical data was analyzed by ANOVA and chi-square tests. The metabolomic dataset was analyzed by multivariate statistics, and the significance of biomarkers was confirmed by ANOVA.Results Clinical data showed differences regarding follicles production, oocyte and embryo quality. From the 15 proposed biomarkers, 14 were of increased abundance in the control group and attributed as fatty acids, diacylglycerol, triacylglycerol, ceramide, ceramide-phosphate, phosphatidylcholine, and sphingomyelin. The PCOS patients showed increased abundance of a metabolite of m/z 144.0023 that was not attributed to a class.Conclusion The clinical and metabolic similarities observed in the FF of hyper responders with and without PCOS diagnosis indicate common biomarkers that could assist on the development of accessory tools for assessment of IVF parameters.