Human monocyte/macrophages activate by exposure to LPS overexpress NGF and NGF receptors
Human monocyte/macrophages activate by exposure to LPS overexpress NGF and NGF receptors
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DOI：
10.1016/s0165-5728(00)00441-0
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发表时间：
2001-02-15
影响因子：
3.3
通讯作者：
Aloe, L
中科院分区：
文献类型：
--
作者：
Caroleo, MC;Costa, N;Aloe, L
单核细胞/巨噬细胞（M/M）是参与炎症反应的免疫系统的主要细胞成分。在本研究中，我们调查了NGF是否由M/M产生并参与了这一事件。结果表明，未受刺激的人M/M产生NGF，其合成受到LPS的刺激。NGF的增加与高亲和力NGF受体在M/M上的表达增强有关，而低亲和力NGF受体的表达没有变化（p75）。在lps活化的M/M中，NGF抗体对内源性NGF进行中和，导致p75受体过表达导致细胞凋亡。这些发现为炎症组织中单核细胞存活的机制提供了新的见解，代表了宿主防御和维持体内平衡的关键方面。(C) 2001 Elsevier Science B.V.版权所有
Monocyte/macrophages (M/M) represent the main cellular component of the immune system involved in the inflammatory response. in the present study we investigate whether NGF is produced by M/M and is involved in this event. The results show that unstimulated human M/M produce NGF and its synthesis is stimulated by LPS. The increase of NGF is associated with enhanced expression of high affinity NGF receptor on M/M and with no changes of low affinity NGF receptors (p75). The neutralization of endogenous NGF by NGF antibody in LPS-activated M/M, leads to overexpression of p75 receptor causing apoptosis. These findings provide new insight in the mechanisms governing monocyte survival in the inflamed tissue, representing a crucial aspect of host defence and maintenance of homeostasis. (C) 2001 Elsevier Science B.V. All rights reserved.