MOLECULAR VEHICLE PROPERTIES OF BROAD HOST RANGE PLASMID-RK2
MOLECULAR VEHICLE PROPERTIES OF BROAD HOST RANGE PLASMID-RK2
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DOI：
10.1126/science.1060178
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发表时间：
1975-01-01
期刊：
影响因子：
56.9
通讯作者：
HELINSKI, DR
中科院分区：
文献类型：
--
作者：
MEYER, R;FIGURSKI, D;HELINSKI, DR
质粒 RK2 在多种革兰氏阴性细菌中稳定存在。 RK2 DNA 有一个 Eco RI 限制位点。将 DNA 片段插入该位点不会干扰质粒的维持或自我传播。由于 RK2 具有广泛的宿主范围，因此它应该可用于体外构建能够通过接合转移或转化到许多革兰氏阴性生物体中而建立的杂合质粒分子。
The plasmid RK2 is stably maintained in a broad range of gram-negative bacteria. The RK2 DNA has a single Eco RI restriction site. The insertion of a DNA fragment into this site does not interfere with either plasmid maintenance or self-transmissibility. Because RK2 has a broad host range, it should be useful for the construction in vitro of hybrid plasmid molecules capable of being established by conjugal transfer or transformation into many genera of gram-negative organisms.