Phylogenetic utility of two existing and four novel nuclear gene loci in reconstructing Tree of Life of ray-finned fishes: The order Cypriniformes (Ostariophysi) as a case study
Phylogenetic utility of two existing and four novel nuclear gene loci in reconstructing Tree of Life of ray-finned fishes: The order Cypriniformes (Ostariophysi) as a case study
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DOI：
10.1016/j.gene.2008.07.016
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发表时间：
2008-11-01
期刊：
影响因子：
3.5
通讯作者：
Mayden, Richard L.
中科院分区：
文献类型：
--
作者：
Chen, Wei-Jen;Miya, Masaki;Mayden, Richard L.
随着近年来多个全基因组计划的完成和公共基因数据库的显著增加，后基因组分析的结果有助于更好地理解生物多样性和基因功能复杂性背后的基因组进化。基因组信息和资源的大量涌入也有利于系统生物学学科的发展。在本文中，我们描述了一组6个以前的和22个新的PCR/测序引物RAG 1，视紫红质和四个新的核标记IRBP，EGR 1，EGR 2B和EGR 3，我们开发了一种方法，利用公共遗传/基因组数据挖掘正在进行的生命树项目之一，旨在了解地球上最大的淡水鱼类的进化关系-鲤形目。本文介绍的引物和实验室方案在33种鲤中成功地进行了测试，包括所有鲤形目的家庭和亚科群体。对每个基因的系统发育表现及其在鲤科鱼类进化研究中的意义进行了评价和讨论。(c)2008 Elsevier B. V.保留所有权利。
After the completion of several entire genome projects and a remarkable increase in public genetic databases in the recent years the results of post-genomic analyses can facilitate a better understanding of the genomic evolution underlying the diversity of organisms and the complexity of gene function. This influx of genomic information and resources is also beneficial to the discipline of systematic biology. In this paper, we describe a set of 6 previous and 22 new PCR/sequencing primers for RAG1, Rhodopsin and four novel nuclear markers from IRBP, EGR1, EGR2B and EGR3 that we developed through an approach making use of public genetic/genomic data mining for one of the ongoing tree of life projects aimed at understanding the evolutionary relationships of the planet's largest clade of freshwater fishes - the Cypriniformes. The primers and laboratory protocols presented here were successfully tested in 33 species comprising all cypriniform family and subfamily groups. Phylogenetic performance of each gene, as well as their implications in the investigation of the evolution of cypriniform fishes were assessed and discussed. (c) 2008 Elsevier B.V. All rights reserved.