A Serological and Molecular Survey of Hepatitis B in Children 15 Years After Inception of the National Hepatitis B Vaccination Program in Eastern China
A Serological and Molecular Survey of Hepatitis B in Children 15 Years After Inception of the National Hepatitis B Vaccination Program in Eastern China
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DOI：
10.1002/jmv.21522
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发表时间：
2009-09-01
影响因子：
12.7
通讯作者：
Ruan, Bing
中科院分区：
文献类型：
--
作者：
Dong, Ying;Liu, She-Lan;Ruan, Bing
自从世界范围内引入有效的B型肝炎疫苗以来，B型肝炎病毒（HBV）S基因突变的出现已经威胁到疫苗接种计划的长期成功。本研究于2007年在中国东部地区对5，407名儿童（0-8岁）进行了研究。我们通过微粒子酶免疫分析、PCR和DNASTAR软件分析了HBsAg、抗-HBs和HBV S基因中“a”决定簇突变的流行情况。儿童HBsAg总检出率为1.52%（82/5,407），随年龄增长而增高。而抗-HBs阳性率（65.42%，2,374/3,629）和滴度随年龄增长而下降。主要感染是HBV C基因型和adr血清型（45/51; 88%的病例）。在儿童中发现I126 T、137位氨基酸（nt 553 T缺失突变）、G145 A、G145 R和F158 S突变; 137位氨基酸和F158 S突变以前未见报道。突变株总检出率为14%（7/51）。为了调查感染是否由母体传播引起，我们比较了16对母子的S基因序列。14对母子表现出相同的HBV基因型，S基因的序列同源性为99.5-100%，而2对表现出不同的基因型。这项研究表明，中国东部地区的B型肝炎疫苗接种策略是成功的。尽管HBV S基因中“a”决定簇突变的出现导致了免疫儿童的HBV感染，但这并不对疫苗接种策略构成威胁。HBV感染儿童通过垂直传播感染。J. Med. Virol. 81：1517-1524，2009. (C)2009 Wiley-Liss，Inc.
The emergence of mutations in the hepatitis B virus (HBV) S gene has threatened the long-term success of vaccination programs since the worldwide introduction of effective vaccines against hepatitis B. This study was conducted on 5,407 children (0-8 years old) in eastern China in 2007. We analyzed the prevalence of HBsAg, anti-HBs, and "a"-determinant mutations in the HBV S gene by microparticle enzyme immunoassays, PCR, and DNASTAR software. The total HBsAg prevalence was 1.52% (82/5,407) in the children and increased with age. In contrast, the positive rate (65.42%, 2,374/3,629) and the titers of anti-HBs decreased with age. The predominant infection was HBV of genotype C and serotype adr (45/51; 88% of cases). Mutations of I126T, amino acid 137 (nt553T deletion mutation), G145A, G145R, and F158S were found in the children; the mutations of amino acid 137 and F158S have not been reported previously. The total prevalence of mutant strains was 14% (7/51). To investigate whether the infection resulted from maternal transmission, we compared the S gene sequences in 16 mother-child pairs. Fourteen mother-child pairs exhibited the same HBV genotype, with 99.5-100% sequence homology in the S gene, while two pairs exhibited different genotypes. This study suggested that the hepatitis B vaccination strategies in eastern China have been successful. Although the emergence of "a"-determinant mutations in the HBV S gene have resulted in HBV infection in immunized children, this does not pose a threat to the vaccination strategies. The HBV-infected children had contracted the infection via vertical transmission. J. Med. Virol. 81:1517-1524, 2009. (C) 2009 Wiley-Liss, Inc.