PEROXISOMES IN HUMAN COLON CARCINOMAS - A CYTOCHEMICAL AND BIOCHEMICAL-STUDY
PEROXISOMES IN HUMAN COLON CARCINOMAS - A CYTOCHEMICAL AND BIOCHEMICAL-STUDY
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DOI：
10.1007/bf02899685
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发表时间：
1992-09-01
期刊：
影响因子：
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通讯作者：
DAUCA, M
中科院分区：
文献类型：
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作者：
CABLE, S;KELLER, JM;DAUCA, M
用超微结构水平的细胞化学研究和生化分析，对正常人和肿瘤人结肠上皮细胞中过氧化物体的存在及其酶含量进行了研究和比较。正常结肠组织中过氧化氢酶阳性细胞器的数量明显多于结肠肿瘤细胞。生化检测显示，正常组织和肿瘤组织中均未检测到D-氨基酸氧化酶和L-α-羟基酸氧化酶活性。与对照组织相比，癌细胞中过氧化氢酶、脂肪酰辅酶A氧化酶和Enoyl-CoA水合酶/3-羟基酰辅酶A脱氢酶(β-氧化系统的过氧化物体双功能酶)的比活性降低。根据Dukes的说法，过氧化氢酶活性的下降与肿瘤的分期有很好的相关性，这表明这种过氧化物酶可以作为一个潜在的预后标志。
The presence of peroxisomes and their enzymic content were investigated and compared in healthy and neoplastic human colon epithelial cells using cytochemical studies at the ultrastructural level as well as biochemical analyses. Catalase-positive organelles were found to be more numerous in normal than in colonic neoplastic cells. Biochemical assays revealed that no D-aminoacid oxidase or L-alpha-hydroxyacid oxidase activity was detected in normal or tumor tissues. The specific activities of catalase, fatty-acyl CoA oxidase and enoyl-CoA hydratase/3 hydroxyacyl-CoA dehydrogenase (the so-called peroxisomal bifunctional enzyme of the beta-oxidation system) were found to be diminished in carcinoma cells compared with the control tissue. The fall in catalase activity correlated well with tumor stage according to Dukes, suggesting that this peroxisomal enzyme could be used as a potential prognostic marker.