Reduced cholesterol transmucosal transport in rats with inhibited mucosal acyl CoA:cholesterol acyltransferase and normal pancreatic function.
Reduced cholesterol transmucosal transport in rats with inhibited mucosal acyl CoA:cholesterol acyltransferase and normal pancreatic function.
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发表时间：
1984-02
影响因子：
6.5
通讯作者：
S. Clark;A. Tercyak
中科院分区：
文献类型：
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作者：
S. Clark;A. Tercyak
在胰腺功能正常的肠系膜淋巴瘘大鼠中研究了粘膜酰基辅酶A：胆固醇酰基转移酶抑制过程中胆固醇的吸收。使用的特异性抑制剂（Sandoz化合物58-035; 3-（癸基二甲基甲硅烷基）-N-[2-（4-甲基苯基）-1-苯乙基]丙酰胺）在体外大大降低了胆固醇酯化，在体内降低了酯化胆固醇的淋巴分泌，但在体外或体内不影响肠道的甘油三酯代谢。在胆固醇的稳态淋巴转运过程中，未酯化的胆固醇增加，胆固醇酯在全淋巴、淋巴乳糜微粒和极低密度脂蛋白中减少。掺入标记的胆固醇注入到管腔中的淋巴极低密度脂蛋白的胆固醇酯，特别是抑制。标记的胆固醇掺入到个人的胆固醇酯不同，并受到不同的影响时，总胆固醇酯的合成受到抑制。结果支持粘膜酰基辅酶A：胆固醇酰基转移酶在胆固醇吸收中的主要调节作用，并暗示肠粘膜内源性和外源性胆固醇代谢的差异。
Absorption of cholesterol during inhibition of mucosal acyl CoA:cholesterol acyltransferase was studied in mesenteric lymph fistula rats with normal pancreatic function. The specific inhibitor used (Sandoz Compound 58-035; 3-(decyldimethylsilyl)-N-[2-(4-methylphenyl)-1-phenylethyl]prop anamide) greatly reduced cholesterol esterification in vitro and decreased lymphatic secretion of esterified cholesterol in vivo, but did not affect triglyceride metabolism by the gut in vitro or in vivo. During steady state lymphatic transport of cholesterol, unesterified cholesterol was increased and cholesteryl esters were decreased in whole lymph, lymph chylomicrons, and very low density lipoproteins. Incorporation of labeled cholesterol infused into the lumen into cholesteryl esters of lymph very low density lipoproteins was particularly suppressed. Labeled cholesterol incorporation into individual cholesteryl esters differed and was differentially affected when total cholesteryl ester synthesis was inhibited. The results support a major regulatory role for mucosal acyl CoA:cholesterol acyltransferase in cholesterol absorption and imply differences in the metabolism of endogenous and exogenous cholesterol by the intestinal mucosa.