Unbiased genomic distribution of genes related to cell morphogenesis in cotton by chromosome mapping
Unbiased genomic distribution of genes related to cell morphogenesis in cotton by chromosome mapping
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DOI：
10.1007/s11240-011-0059-8
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发表时间：
2012-03-01
影响因子：
3
通讯作者：
Zhang, Xianlong
中科院分区：
文献类型：
--
作者：
Liu, Chuanxiang;Lin, Zhongxu;Zhang, Xianlong
细胞去分化、体细胞胚胎发生和细胞壁再生是植物再生的关键步骤。为了提高棉花植物再生的效率，我们绘制了与细胞形态发生相关的基因图谱。总共开发了 489 个标记，包括 SSR、PIP 以及与细胞去分化、体细胞胚胎发生和细胞壁再生相关的序列特异性标记。根据高分辨率凝胶和 SSCP 分析，只有 19 个标记显示作图群体亲本之间的多态性，并生成了 21 个多态性位点。 9 个棉花染色体上有 13 个位点，其中 4 个位点位于 Chr16 上。 13 个基因座中的 7 个位于 At 亚基因组上，6 个位于 Dt 亚基因组上。本研究概述了棉花细胞形态发生相关基因的染色体分布。本研究开发的标记将有助于标记辅助选择棉花植物再生的更好基因型。
Cell dedifferentiation, somatic embryogenesis, and cell wall regeneration are key steps in plant regeneration. In order to improve the efficiency of plant regeneration in cotton, we mapped genes related to cell morphogenesis. A total of 489 markers, including SSRs, PIPs, and sequence-specific markers related to cell dedifferentiation, somatic embryogenesis, and cell wall regeneration were developed. Only 19 markers showed polymorphism between parents of the mapping population upon high-resolution gel and SSCP analysis, and 21 polymorphic loci were generated. Thirteen loci were mapped on 9 cotton chromosomes, four of which were on Chr16. Seven of the 13 loci were mapped on the At sub-genome and six on the Dt sub-genome. This study provides an overview of the chromosome distribution of genes related to cell morphogenesis in cotton. The markers developed in this study will be useful in marker-assisted selection of better genotypes for plant regeneration in cotton.