In vitro transcytosis of Helicobacter pylori histidine-rich protein through gastric epithelial-like cells and the blood?brain barrier
In vitro transcytosis of Helicobacter pylori histidine-rich protein through gastric epithelial-like cells and the blood?brain barrier
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幽门螺杆菌富含组氨酸蛋白的体外转胞吞作用通过胃上皮样细胞和血脑屏障
DOI：
10.1093/bbb/zbab221
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Kawano Tsuyoshi
中科院分区：
文献类型：
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作者：
Iwasaki Takashi;Maruyama Aiki;Inui Yurika;Sakurai Toshihiko;Kawano Tsuyoshi
最近的流行病学研究支持幽门螺杆菌感染与阿尔茨海默病的发展之间的相关性。HpHpn，富含组氨酸的H.幽门螺杆菌蛋白，形成淀粉样寡聚体；它可能是阿尔茨海默病进展的致病因素。HPHpn也可从胃上皮转运到脑内。然而，HpHpn是由h分泌的。幽门位于胃上皮细胞的外表面；因此，假设HPHpn通过胃上皮到血液中的移动仍然存在争议。在这里，我们发现HPHpn在人胃上皮样癌细胞中表现出酸性的pH依赖的细胞摄取和随后的分泌。此外，HPHpn还表现出对血脑屏障的体外通透性。虽然还需要进一步的活体实验，但我们的发现表明，胃上皮细胞和血脑屏障中HPHpn的体外跨细胞作用可能为H之间的相关性提供新的见解。幽门螺杆菌感染和阿尔茨海默病的进展。
Recent epidemiological studies have supported the correlation betweenHelicobacter pyloriinfection and the development of Alzheimer’s disease. HpHpn, a histidine-richH. pyloriprotein, forms amyloid-like oligomers; it may be a pathogenic factor for Alzheimer’s disease progression. HpHpn may also be transported from the gastric epithelium to the brain. However, HpHpn is secreted fromH. pylorion the outer surface of gastric epithelia; therefore, the hypothesized movement of HpHpn across the gastric epithelium to the blood remains controversial. Here, we found the HpHpn showed acidic pH-dependent cellular uptake and subsequent secretion in human gastric epithelial-like carcinoma cells. Furthermore, HpHpn exhibitedin vitropermeability across the blood–brain barrier. Although furtherin vivoexperiments are required, our findings suggest thatin vitrotranscytosis of HpHpn in gastric epithelial cells and the blood–brain barrier may provide new insights into the correlation betweenH. pyloriinfections and Alzheimer’s disease progression.