EFFECT OF COMPONENTS OF ACID-HYDROLYZED HARDWOOD ON CONVERSION OF D-XYLOSE TO 2,3-BUTANEDIOL BY KLEBSIELLA-PNEUMONIAE

EFFECT OF COMPONENTS OF ACID-HYDROLYZED HARDWOOD ON CONVERSION OF D-XYLOSE TO 2,3-BUTANEDIOL BY KLEBSIELLA-PNEUMONIAE
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DOI:
10.1016/0141-0229(91)90164-6
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发表时间:
1991-02-01
影响因子:
3.4
通讯作者:
MCCASKEY, TA
MCCASKEY, TA
中科院分区:
工程技术3区
文献类型:
--
作者:
FRAZER, FR;MCCASKEY, TA

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研究了不同酸解阔叶木(水解液)组分对一株从纤维废弃物中分离的肺炎克雷伯氏菌生长、糖分利用和2,3-丁二醇(BDO)产量的影响。醋酸盐、硫酸盐、糠醛和酚类化合物被评估为常见于水解物中的潜在微生物抑制剂。根据接种水平的不同,最高可达1.6%(w/v)的乙酸乙酯对BDO的产量有促进作用。高达0.2%(w/v)的硫酸盐水平不会影响生长,但会使BDO产量减少约30%。当糠醛浓度达到0.2%(w/v)时,也获得了类似的生长结果,但BDO的产率略高。在所评价的潜在抑制剂中,酚类化合物(丁香醛和香草醛)对生长的抑制作用最强。0.10%(w/v)的浓度对BDO的生长有抑制作用,0.05%(w/v)的浓度则抑制BDO的产生。
The effects of selected components of acid-hydrolysed hardwood (hydrolysate) on growth, sugar utilization, and 2,3-butanediol (BDO) production by a strain of Klebsiella pneumoniae isolated from cellulosic waste were investigated. Acetate, sulfate, furfural, and phenolic compounds were evaluated as potential microbial inhibitors commonly present in hydrolysate. Depending on the inoculation level, yields of BDO were stimulated by acetate levels up to 1.6% (w/v). Sulfate levels up to 0.2% (w/v) did not affect growth, but reduced the BDO yield by approximately 30%. Similar growth results were obtained with furfural concentrations up to 0.2% (w/v), but BDO yields were slightly higher. Phenolic compounds (syringealdehyde and vanillin) were the most growth inhibitory of the potential inhibitors evaluated. Growth was inhibited by concentrations of 0.10% (w/v), while concentrations as low as 0.05% (w/v) inhibited BDO production.