Evaluation of adult equine bone marrow- and adipose-derived progenitor cell chondrogenesis in hydrogel cultures
Evaluation of adult equine bone marrow- and adipose-derived progenitor cell chondrogenesis in hydrogel cultures
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DOI：
10.1002/jor.20508
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发表时间：
2008-03-01
影响因子：
2.8
通讯作者：
Frisbie, David D.
中科院分区：
文献类型：
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作者：
Kisiday, John D.;Kopesky, Paul W.;Frisbie, David D.
骨髓间充质干细胞（BM-MSCs）和脂肪源性祖细胞（ADPC）是软骨表面重建策略中自体软骨细胞的潜在替代品。在这项研究中，通过定量新组织合成和测定软骨的细胞外基质（ECM）成分的基因表达和积累，比较了这些细胞类型的软骨形成潜力。将包封在琼脂糖或自组装肽水凝胶中的成年马祖细胞在存在或不存在TGF β 1的情况下培养3周。在BM-MSC接种的水凝胶中，TGF β 1刺激EGM合成和积累，相对于无TGF β 1的培养物是3-41倍。在ADPC培养物中，TGF β 1刺激ECM合成和肽中积累的显著增加（18-29倍），但不是琼脂糖水凝胶。在TGF β 1培养基中培养的BM-MSC接种的琼脂糖和肽水凝胶的色谱分析显示聚集蛋白聚糖样蛋白聚糖单体的广泛合成。接种在肽水凝胶中的ADPC也合成聚集蛋白聚糖样蛋白聚糖，尽管程度低于BM-MSC水凝胶中所见，而ADPC接种琼脂糖中的聚集蛋白聚糖样蛋白聚糖合成最少。TGF β 1培养物的RT-PCR分析显示BM-MSC中可检测到II型胶原基因表达水平，但ADPC培养物中没有。TGF β 1-培养的肽水凝胶的组织学分析显示，BM-MSC而不是ADPC沉积了连续的富含蛋白多糖和II型胶原的ECM。因此，本研究显示了BM-MSC相对于ADPC的上级软骨形成的蛋白质和基因表达证据。(C)2007骨科研究学会。出版社：Wiley Periodicals，Inc.
Bone marrow mesenchymal stem cells (BM-MSCs) and adipose-derived progenitor cells (ADPCs) are potential alternatives to autologous chondrocytes for cartilage resurfacing strategies. In this study, the chondrogenic potentials of these cell types were compared by quantifying neo-tissue synthesis and assaying gene expression and accumulation of extracellular matrix (ECM) components of cartilage. Adult equine progenitor cells encapsulated in agarose or self-assembling peptide hydrogels were cultured in the presence or absence of TGF beta 1 for 3 weeks. In BM-MSCs-seeded hydrogels, TGF beta 1 stimulated EGM synthesis and accumulation 3-41-fold relative to TGF beta 1-free culture. In ADPC cultures, TGF beta 1 stimulated a significant increase in ECM synthesis and accumulation in peptide (18-29-fold) but not agarose hydrogels. Chromatographic analysis of BM-MSC-seeded agarose and peptide hydrogels cultured in TGF beta 1 medium showed extensive synthesis of aggrecan-like proteoglycan monomers. ADPCs seeded in peptide hydrogel also synthesized aggrecan-like proteoglycans, although to a lesser extent than seen in BM-MSC hydrogels, whereas aggrecan-like proteoglycan synthesis in ADPC-seeded agarose was minimal. RT-PCR analysis of TGF beta 1 cultures showed detectable levels of type II collagen gene expression in BM-MSC but not ADPC cultures. Histological analysis of TGF beta 1-cultured peptide hydrogels showed the deposition of a continuous proteoglycan- and type II collagen rich ECM for BM-MSCs but not ADPCs. Therefore, this study showed both protein and gene expression evidence of superior chondrogenesis of BM-MSCs relative to ADPCs. (C) 2007 Orthopaedic Research Society. Published by Wiley Periodicals, Inc.