NTPDase1 and NTPDase2 Immunolocalization in Mouse Cochlea: Implications for Regulation of P2 Receptor Signaling
NTPDase1 and NTPDase2 Immunolocalization in Mouse Cochlea: Implications for Regulation of P2 Receptor Signaling
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小鼠耳蜗中的 NTPDase1 和 NTPDase2 免疫定位：对 P2 受体信号传导调节的影响
DOI：
10.1177/002215540205001102
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发表时间：
2002
影响因子：
3.2
通讯作者：
G. Housley
中科院分区：
文献类型：
--
作者：
S. Vlajkovic;P. Thorne;J. Sévigny;S. Robson;G. Housley
细胞、分子和生理研究已经证明ATP和相关核苷酸通过内耳耳蜗P2受体起重要的信号传导作用。信号调节是由外核酶促进的，外核酶是一种异源的表面酶家族，参与细胞外核苷酸水解。我们之前的研究表明CD39/NTPDase1和CD39L1/NTPDase2是外核苷三磷酸二磷酸水解酶（E-NTPDase）家族的成员，是耳蜗淋巴内室和淋巴周围室衬里组织中的主要atp水解酶。ntpdase能水解三磷酸核苷和二磷酸核苷。相反，NTPDase2是一种优先的核苷三磷酸酶。本研究通过免疫组织化学方法表征了这些e - ntpases在小鼠耳蜗中的表达。NTPDase1可以免疫定位到耳蜗血管和神经组织（螺旋神经节的初级听觉神经元）。相反，NTPDase2免疫标记主要定位于感觉内外毛细胞的突触区、外毛细胞的立纤毛和角质层板、Corti器官的支持细胞（Deiters细胞和内缘细胞）、位于节内螺旋束的传出神经纤维、螺旋韧带的外沟和根区。耳蜗中NTPDase1和2的差异表达表明P2受体信号的空间调控可能涉及不同的核苷酸种类和水解动力学。
Cellular, molecular, and physiological studies have demonstrated an important signaling role for ATP and related nucleotides acting via P2 receptors in the cochlea of the inner ear. Signal modulation is facilitated by ectonucleotidases, a heterologous family of surface-located enzymes involved in extracellular nucleotide hydrolysis. Our previous studies have implicated CD39/NTPDase1 and CD39L1/NTPDase2, members of the ectonucleoside triphosphate diphosphohydrolase (E-NTPDase) family, as major ATP-hydrolyzing enzymes in the tissues lining the cochlear endolymphatic and perilymphatic compartments. NTPDase1 hydrolyzes both nucleoside triphosphates and diphosphates. In contrast, NTPDase2 is a preferential nucleoside triphosphatase. This study characterizes expression of these E-NTPDases in the mouse cochlea by immunohistochemistry. NTPDase1 can be immunolocalized to the cochlear vasculature and neural tissues (primary auditory neurons in the spiral ganglion). In contrast, NTPDase2 immunolabeling was principally localized to synaptic regions of the sensory inner and outer hair cells, stereocilia and cuticular plates of the outer hair cells, supporting cells of the organ of Corti (Deiters’ cells and inner border cells), efferent nerve fibers located in the intraganglionic spiral bundle, and in the outer sulcus and root region of the spiral ligament. This differential expression of NTPDase1 and 2 in the cochlea suggests spatial regulation of P2 receptor signaling, potentially involving different nucleotide species and hydrolysis kinetics.
DOI：
10.1046/j.1432-1033.2001.01896.x
发表时间：
2001
期刊：
European journal of biochemistry
影响因子：
--
作者：
P. Heine;N. Braun;Jean Sévigny;Simon C. Robson;Jörg Servos;Herbert Zimmermann
通讯作者：
P. Heine;N. Braun;Jean Sévigny;Simon C. Robson;Jörg Servos;Herbert Zimmermann
声学和电学听力中的响度适应。
DOI：
10.1007/s10162-005-0023-6
发表时间：
2006
期刊：
Journal of the Association for Research in Otolaryngology : JARO
影响因子：
--
作者：
Tang,Qing;Liu,Sheng;Zeng,Fan-Gang
通讯作者：
Zeng,Fan-Gang
一氧化氮/环 GMP 途径可减弱豚鼠耳蜗支持细胞中 ATP 引起的细胞内钙增加。
DOI：
--
发表时间：
2000
期刊：
The Journal of comparative neurology.
影响因子：
--
作者：
Matsunobu,T;Schacht,J
通讯作者：
Schacht,J