Global transcriptional profile of Mycobacterium tuberculosis during THP-1 human macrophage infection
Global transcriptional profile of Mycobacterium tuberculosis during THP-1 human macrophage infection
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DOI：
10.1128/iai.00974-07
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发表时间：
2008-02-01
影响因子：
3.1
通讯作者：
Smith, Issar
中科院分区：
文献类型：
--
作者：
Fontan, Patricia;Aris, Virginie;Smith, Issar
在肺部感染期间，结核分枝杆菌驻留在巨噬细胞中，并破坏这些专职吞噬细胞的杀菌机制。理解这种宿主-病原体关系对于开发治疗和预防结核病的新疗法至关重要。在这项工作中，我们分析了M。感染人巨噬细胞样THP-1细胞的结核分枝杆菌，以鉴定可能与M.结核我们比较了M.在感染人巨噬细胞样THP-1细胞4小时和24小时后，结核病H37 Rv菌株的基因表达谱在肉汤培养物中呈指数增长。我们发现585个基因在M.结核对M. THP-1细胞内的结核表明细胞包膜的扰动是THP-1巨噬细胞内的主要细胞内应激。
During lung infection, Mycobacterium tuberculosis resides in macrophages and subverts the bactericidal mechanisms of these professional phagocytes. Comprehension of this host-pathogen relationship is fundamental for the development of new therapies to cure and prevent tuberculosis. In this work, we analyzed the transcriptional profile of M. tuberculosis infecting human macrophage-like THP-1 cells in order to identify putative bacterial pathogenic factors that can be relevant for the intracellular survival of M. tuberculosis. We compared the gene expression profile of M. tuberculosis H37Rv after 4 h and 24 h of infection of human macrophage-like THP-1 cells with the gene expression profile of the strain growing exponentially in broth cultures. We found 585 genes expressed differentially by intracellular M. tuberculosis. An analysis of the gene expression profile of M. tuberculosis inside THP-1 cells suggests the perturbation of the cell envelope as a major intracellular stress inside THP-1 macrophages.