3,6-O-[N-(2-Aminoethyl)-acetamide-yl]-chitosan exerts antibacterial activity by a membrane damage mechanism.
3,6-O-[N-(2-Aminoethyl)-acetamide-yl]-chitosan exerts antibacterial activity by a membrane damage mechanism.
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DOI：
10.1016/j.carbpol.2016.04.098
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发表时间：
2016-09
影响因子：
11.2
通讯作者：
Feilong Yan;Qifeng Dang;Chengsheng Liu;Jingquan Yan;Teng Wang;Bing Fan;Dongsu Cha;Xiaoli Li;Shengnan Liang;Zhenzhen Zhang
中科院分区：
文献类型：
--
作者：
Feilong Yan;Qifeng Dang;Chengsheng Liu;Jingquan Yan;Teng Wang;Bing Fan;Dongsu Cha;Xiaoli Li;Shengnan Liang;Zhenzhen Zhang
制备了一种新型壳聚糖衍生物3,6- o -[N-（2-氨基乙基）-乙酰胺基]壳聚糖（AACS），以改善壳聚糖的水溶性和抗菌活性。AACS对大肠杆菌和金黄色葡萄球菌具有良好的抑菌活性，最低抑菌浓度为0.25 mg/mL。细胞膜完整性、电导率和NPN摄取试验表明，AACS通过破坏细胞膜完整性导致细胞内核酸释放、NPN摄取和电导率迅速增加。另一方面，疏水性、细胞活力和SDS-PAGE实验表明，AACS可以通过增加膜透性来降低表面疏水性、细胞活力和细胞内蛋白。SEM观察进一步证实了AACS可以通过破坏细菌的膜来杀死细菌。以上结果验证了AACS主要通过膜损伤机制发挥抑菌作用，有望成为一种新型的食品防腐剂。
A novel chitosan derivative, 3,6-O-[N-(2-aminoethyl)-acetamide-yl]-chitosan (AACS), was successfully prepared to improve water solubility and antibacterial activity of chitosan. AACS had good antibacterial activity, with minimum inhibitory concentrations of 0.25 mg/mL, againstEscherichia coliandStaphylococcus aureus. Cell membrane integrity, electric conductivity and NPN uptake tests showed that AACS caused quickly increasing the release of intracellular nucleic acids, the uptake of NPN, and the electric conductivity by damaging membrane integrity. On the other hand, hydrophobicity, cell viability and SDS-PAGE experiments indicated that AACS was able to reduce the surface hydrophobicity, the cell viability and the intracellular proteins through increasing membrane permeability. SEM observation further confirmed that AACS could kill bacteria via disrupting their membranes. All results above verified that AACS mainly exerted antibacterial activity by a membrane damage mechanism, and it was expected to be a new food preservative.