Application of Multiphoton Microscopy in Dermatological Studies: a Mini-Review.
Application of Multiphoton Microscopy in Dermatological Studies: a Mini-Review.
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DOI：
10.1142/s1793545813300103
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发表时间：
2014-01-03
影响因子：
2.5
通讯作者：
So PT
中科院分区：
文献类型：
--
作者：
Yew E;Rowlands C;So PT
本文综述了多光子显微镜在皮肤病学中的应用的历史和最新进展。与其他显微镜技术相比，多光子显微镜有几个优势：固有的轴向切片，由于使用近红外光，穿透深度与共聚焦显微镜竞争得很好，许多双光子对比机制，如二次谐波产生，在单光子显微镜中没有类似物。虽然光子进入组织的穿透深度通常被限制在数百微米的数量级，但这在皮肤病学中不太受关注，因为皮肤很薄，很容易接触到。因此，多光子显微镜在皮肤病学已经产生了很大的兴趣，其中大部分是总结在这里。这篇综述涵盖了光和组织的相互作用，以及在设计仪器时必须考虑的各种因素。本文还讨论了多光子显微镜成像皮肤癌和其他各种疾病的现状，以及衰老和再生现象的研究，最后总结了利用多光子显微镜分析药物、化妆品和其他药物的透皮转运。该综述最后展望了未来潜在的研究方向，特别是那些将这些技术推向广泛临床接受所必需的方向。
This review summarizes the historical and more recent developments of multiphoton microscopy, as applied to dermatology. Multiphoton microscopy offers several advantages over competing microscopy techniques: there is an inherent axial sectioning, penetration depths that compete well with confocal microscopy on account of the use of near-infrared light, and many two-photon contrast mechanisms, such as second-harmonic generation, have no analogue in one-photon microscopy. While the penetration depths of photons into tissue are typically limited on the order of hundreds of microns, this is of less concern in dermatology, as the skin is thin and readily accessible. As a result, multiphoton microscopy in dermatology has generated a great deal of interest, much of which is summarized here. The review covers the interaction of light and tissue, as well as the various considerations that must be made when designing an instrument. The state of multiphoton microscopy in imaging skin cancer and various other diseases is also discussed, along with the investigation of aging and regeneration phenomena, and finally, the use of multiphoton microscopy to analyze the transdermal transport of drugs, cosmetics and other agents is summarized. The review concludes with a look at potential future research directions, especially those that are necessary to push these techniques into widespread clinical acceptance.