Investigating Rhodamine B-Labeled Peptoids: Scopes and Limitations of Its Applications
Investigating Rhodamine B-Labeled Peptoids: Scopes and Limitations of Its Applications
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DOI：
10.1002/bip.21617
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发表时间：
2011-01-01
期刊：
影响因子：
2.9
通讯作者：
Braese, Stefan
中科院分区：
文献类型：
--
作者：
Birtalan, Esther;Rudat, Birgit;Braese, Stefan
荧光载体罗丹明B常用于生物检测。它价格低廉，在各种反应条件下都很稳定，可以共价连接到生物活性分子上，并且在吸收和荧光波长方面具有合适的光谱性质。尽管如此，它也有一些缺点：它很容易形成螺内酰胺化合物，这是非荧光的，因此可能不是所有荧光显微镜应用的首选染料。在这里，这种螺内酰胺的形成是通过用高效液相色谱(HPLC)纯化这种标记的类肽来观察的，并通过进行一系列随时间推移的分析性高效液相色谱进行详细的监测。此外，还合成了以罗丹明B为标记物的8个类肽的小文库。对这些分子的吸收特性的分析表明，可以通过将仲胺与罗丹明B偶联来克服荧光损失的问题。(C)2011 Wiley Percials，Inc.生物聚合物(Pept Sci)96：694-701,2011。
The fluorophore rhodamine B is often used in biological assays. It is inexpensive, robust under a variety of reaction conditions, can be covalently linked to bioactive molecules, and has suitable spectral properties in terms of absorption and fluorescence wavelength. Nonetheless, there are some drawbacks: it can readily form a spirolactam compound, which is nonfluorescent, and therefore may not be the dye of choice for all fluorescence microscopy applications. Herein this spirolactam formation was observed by purifying such a labeled peptoid with high performance liquid chromatography (HPLC) and monitored in detail by making a series of analytical HPLC runs over time. Additionally, a small library of eight peptoids with rhodamine B as label was synthesized. Analysis of the absorption properties of these molecules demonstrated that the problem of fluorescence loss can be overcome by coupling secondary amines with rhodamine B. (C) 2011 Wiley Periodicals, Inc. Biopolymers (Pept Sci) 96: 694-701, 2011.